TY  - JOUR
AU  - Guenther, Anne
AU  - Luczak, Vince
AU  - Abel, Ted
AU  - Baumann, Arnd
TI  - Caspase-3 and GFAP as early markers for apoptosis and astrogliosis in shRNA-induced hippocampal cytotoxicity
JO  - The journal of experimental biology
VL  - 220
IS  - 8
SN  - 1477-9145
CY  - Cambridge
M1  - FZJ-2017-03164
SP  - 1400 - 1404
PY  - 2017
AB  - Genetic manipulation of cells and tissue by RNA interference has significantly contributed to the functional characterization of individual proteins and their role in physiological processes. Despite its versatility, RNA interference can have detrimental side effects, including reduced cell viability. We applied recombinant adeno-associated viruses by stereotaxic injection into the murine hippocampus to express different short hairpin RNA (shRNA) constructs along with eGFP. Tissue responses were assessed immunohistochemically for up to 8 weeks post-infection. Strong hippocampal degeneration and tissue atrophy was observed, most likely induced by high shRNA expression. The effect was entirely absent in mice injected with vectors driving only expression of eGFP. Active caspase-3 (Casp-3) and glial fibrillary acidic protein (GFAP) were identified as molecular markers and early indicators of adverse tissue responses. Our findings also demonstrate that detrimental effects of high shRNA expression in hippocampal tissue can be monitored even before the onset of tissue degeneration.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000399670800008
DO  - DOI:10.1242/jeb.154583
UR  - https://juser.fz-juelich.de/record/829465
ER  -