TY  - JOUR
AU  - Wingen, Marcus
AU  - Jaeger, Karl-Erich
AU  - Gensch, Thomas
AU  - Drepper, Thomas
TI  - Novel Thermostable Flavin-binding Fluorescent Proteins from Thermophilic Organisms
JO  - Photochemistry and photobiology
VL  - 93
IS  - 3
SN  - 0031-8655
CY  - Malden, Mass.
PB  - Wiley-Blackwell
M1  - FZJ-2017-03599
SP  - 849 - 856
PY  - 2017
AB  - Flavin-binding fluorescent proteins (FbFPs) are small, oxygen-independent in vivo reporters, derived from Light Oxygen Voltage (LOV) domains of photoreceptors. Here, we investigated the thermostability of existing, as well as novel FbFPs, whose genes were identified in genome sequences of various thermophilic bacteria as well as metagenomic libraries from hot springs in the Yellowstone National Park. Detailed in vitro analyses revealed that two of those fluorescent reporter proteins were highly thermostable, exhibiting melting temperatures above 75°C.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000401262000023
C6  - pmid:28500719
DO  - DOI:10.1111/php.12740
UR  - https://juser.fz-juelich.de/record/829997
ER  -