%0 Journal Article
%A Giani, M.
%A den Otter, W. K.
%A Briels, Willem
%T Early stages of clathrin aggregation at a membrane in coarse-grained simulations
%J The journal of chemical physics
%V 146
%N 15
%@ 1089-7690
%C Melville, NY
%I American Institute of Physics
%M FZJ-2017-03611
%P 155102 -
%D 2017
%X The self-assembly process of clathrin coated pits during endocytosis has been simulated by combining and extending coarse grained models of the clathrin triskelion, the adaptor protein AP2, and a flexible network membrane. The AP2’s core, upon binding to membrane and cargo, releases a motif that can bind clathrin. In conditions where the core-membrane-cargo binding is weak, the binding of this motif to clathrin can result in a stable complex. We characterize the conditions and mechanisms resulting in the formation of clathrin lattices that curve the membrane, i.e., clathrin coated pits. The mechanical properties of the AP2 β linker appear crucial to the orientation of the curved clathrin lattice relative to the membrane, with wild-type short linkers giving rise to the inward curving buds enabling endocytosis while long linkers produce upside-down cages and outward curving bulges
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000399904500036
%R 10.1063/1.4979985
%U https://juser.fz-juelich.de/record/830009