TY  - JOUR
AU  - Giani, M.
AU  - den Otter, W. K.
AU  - Briels, Willem
TI  - Early stages of clathrin aggregation at a membrane in coarse-grained simulations
JO  - The journal of chemical physics
VL  - 146
IS  - 15
SN  - 1089-7690
CY  - Melville, NY
PB  - American Institute of Physics
M1  - FZJ-2017-03611
SP  - 155102 -
PY  - 2017
AB  - The self-assembly process of clathrin coated pits during endocytosis has been simulated by combining and extending coarse grained models of the clathrin triskelion, the adaptor protein AP2, and a flexible network membrane. The AP2’s core, upon binding to membrane and cargo, releases a motif that can bind clathrin. In conditions where the core-membrane-cargo binding is weak, the binding of this motif to clathrin can result in a stable complex. We characterize the conditions and mechanisms resulting in the formation of clathrin lattices that curve the membrane, i.e., clathrin coated pits. The mechanical properties of the AP2 β linker appear crucial to the orientation of the curved clathrin lattice relative to the membrane, with wild-type short linkers giving rise to the inward curving buds enabling endocytosis while long linkers produce upside-down cages and outward curving bulges
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000399904500036
DO  - DOI:10.1063/1.4979985
UR  - https://juser.fz-juelich.de/record/830009
ER  -