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@ARTICLE{Lange:830011,
      author       = {Lange, Christian and Lehr, Matthias and Zerulla, Karolin
                      and Ludwig, Petra and Schweitzer, Jens and Polen, Tino and
                      Wendisch, Volker F. and Soppa, Jörg},
      title        = {{E}ffects of {K}asugamycin on the {T}ranslatome of
                      {E}scherichia coli},
      journal      = {PLoS one},
      volume       = {12},
      number       = {1},
      issn         = {1932-6203},
      address      = {Lawrence, Kan.},
      publisher    = {PLoS},
      reportid     = {FZJ-2017-03613},
      pages        = {e0168143 -},
      year         = {2017},
      note         = {Biotechnologie 1},
      abstract     = {It is long known that Kasugamycin inhibits translation of
                      canonical transcripts containing a 5’-UTR with a Shine
                      Dalgarno (SD) motif, but not that of leaderless transcripts.
                      To gain a global overview of the influence of Kasugamycin on
                      translation efficiencies, the changes of the translatome of
                      Escherichia coli induced by a 10 minutes Kasugamycin
                      treatment were quantified. The effect of Kasugamycin
                      differed widely, 102 transcripts were at least twofold more
                      sensitive to Kasugamycin than average, and 137 transcripts
                      were at least twofold more resistant, and there was a more
                      than 100-fold difference between the most resistant and the
                      most sensitive transcript. The 5’-ends of 19 transcripts
                      were determined from treated and untreated cultures, but
                      Kasugamycin resistance did neither correlate with the
                      presence or absence of a SD motif, nor with differences in
                      5’-UTR lengths or GC content. RNA Structure Logos were
                      generated for the 102 Kasugamycin-sensitive and for the 137
                      resistant transcripts. For both groups a short Shine
                      Dalgarno (SD) motif was retrieved, but no specific motifs
                      associated with resistance or sensitivity could be found.
                      Notably, this was also true for the region -3 to -1 upstream
                      of the start codon and the presence of an extended SD motif,
                      which had been proposed to result in Kasugamycin resistance.
                      Comparison of the translatome results with the database
                      RegulonDB showed that the transcript with the highest
                      resistance was leaderless, but no further leaderless
                      transcripts were among the resistant transcripts.
                      Unexpectedly, it was found that translational coupling might
                      be a novel feature that is associated with Kasugamycin
                      resistance. Taken together, Kasugamycin has a profound
                      effect on translational efficiencies of E. coli transcripts,
                      but the mechanism of action is different than previously
                      described.},
      cin          = {IBG-1},
      ddc          = {500},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {581 - Biotechnology (POF3-581)},
      pid          = {G:(DE-HGF)POF3-581},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000391949500007},
      pubmed       = {pmid:28081129},
      doi          = {10.1371/journal.pone.0168143},
      url          = {https://juser.fz-juelich.de/record/830011},
}