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@ARTICLE{Gushchin:834107,
author = {Gushchin, Ivan and Melnikov, Igor and Polovinkin, Vitaly
and Ishchenko, Andrii and Yuzhakova, Anastasia and Buslaev,
Pavel and Bourenkov, Gleb and Grudinin, Sergei and Round,
Ekaterina and Balandin, Taras and Borshchevskiy, Valentin
and Willbold, Dieter and Leonard, Gordon and Büldt, Georg
and Popov, Alexander and Gordeliy, Valentin},
title = {{M}echanism of transmembrane signaling by sensor histidine
kinases},
journal = {Science},
volume = {356},
number = {6342},
issn = {0036-8075},
address = {Washington, DC [u.a.]},
publisher = {American Association for the Advancement of Science},
reportid = {FZJ-2017-04108},
pages = {6345},
year = {2017},
abstract = {One of the major and essential classes of transmembrane
(TM) receptors, present in all domains of life, is sensor
histidine kinases (HKs), parts of two-component signaling
systems (TCS). The structural mechanisms of transmembrane
signaling by these sensors are poorly understood. We present
here crystal structures of the periplasmic sensor domain,
the TM domain and the cytoplasmic HAMP domain of the
Escherichia coli nitrate/nitrite sensor HK NarQ in the
ligand-bound and mutated ligand-free states. The structures
reveal that the ligand binding induces significant
rearrangements and piston-like shifts of TM helices. The
HAMP domain protomers undergo lever-like motions and convert
the piston-like motions into helical rotations. Our findings
provide the structural framework for complete understanding
of TM TCS signaling and for development of antimicrobial
treatments targeting TCS.},
cin = {ICS-6},
ddc = {500},
cid = {I:(DE-Juel1)ICS-6-20110106},
pnm = {551 - Functional Macromolecules and Complexes (POF3-551)},
pid = {G:(DE-HGF)POF3-551},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000402871700044},
pubmed = {pmid:28522691},
doi = {10.1126/science.aah6345},
url = {https://juser.fz-juelich.de/record/834107},
}