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000834108 1001_ $$0P:(DE-HGF)0$$aMelnikov, Igor$$b0
000834108 245__ $$aFast iodide-SAD phasing for high-throughput membrane protein structure determination
000834108 260__ $$aWashington, DC [u.a.]$$bAssoc.$$c2017
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000834108 520__ $$aWe describe a fast, easy, and potentially universal method for the de novo solution of the crystal structures of membrane proteins via iodide–single-wavelength anomalous diffraction (I-SAD). The potential universality of the method is based on a common feature of membrane proteins—the availability at the hydrophobic-hydrophilic interface of positively charged amino acid residues with which iodide strongly interacts. We demonstrate the solution using I-SAD of four crystal structures representing different classes of membrane proteins, including a human G protein–coupled receptor (GPCR), and we show that I-SAD can be applied using data collection strategies based on either standard or serial x-ray crystallography techniques.
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000834108 7001_ $$0P:(DE-HGF)0$$aPolovinkin, Vitaly$$b1
000834108 7001_ $$0P:(DE-Juel1)169220$$aKovalev, Kirill$$b2
000834108 7001_ $$0P:(DE-Juel1)165798$$aGushchin, Ivan$$b3
000834108 7001_ $$0P:(DE-HGF)0$$aShevtsov, Mikhail$$b4
000834108 7001_ $$0P:(DE-Juel1)171538$$aShevchenko, Vitaly$$b5
000834108 7001_ $$0P:(DE-HGF)0$$aMishin, Alexey$$b6
000834108 7001_ $$0P:(DE-Juel1)165628$$aAlekseev, Alexey$$b7
000834108 7001_ $$0P:(DE-HGF)0$$aRodriguez-Valera, Francisco$$b8
000834108 7001_ $$0P:(DE-Juel1)144613$$aBorshchevskiy, Valentin$$b9
000834108 7001_ $$0P:(DE-HGF)0$$aCherezov, Vadim$$b10
000834108 7001_ $$0P:(DE-HGF)0$$aLeonard, Gordon A.$$b11
000834108 7001_ $$0P:(DE-Juel1)131964$$aGordeliy, Valentin$$b12$$eCorresponding author
000834108 7001_ $$0P:(DE-HGF)0$$aPopov, Alexander$$b13
000834108 773__ $$0PERI:(DE-600)2810933-8$$a10.1126/sciadv.1602952$$n5$$pe1602952$$tScience advances$$v3$$x2375-2548$$y2017
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