TY  - JOUR
AU  - Melnikov, Igor
AU  - Polovinkin, Vitaly
AU  - Kovalev, Kirill
AU  - Gushchin, Ivan
AU  - Shevtsov, Mikhail
AU  - Shevchenko, Vitaly
AU  - Mishin, Alexey
AU  - Alekseev, Alexey
AU  - Rodriguez-Valera, Francisco
AU  - Borshchevskiy, Valentin
AU  - Cherezov, Vadim
AU  - Leonard, Gordon A.
AU  - Gordeliy, Valentin
AU  - Popov, Alexander
TI  - Fast iodide-SAD phasing for high-throughput membrane protein structure determination
JO  - Science advances
VL  - 3
IS  - 5
SN  - 2375-2548
CY  - Washington, DC [u.a.]
PB  - Assoc.
M1  - FZJ-2017-04109
SP  - e1602952
PY  - 2017
AB  - We describe a fast, easy, and potentially universal method for the de novo solution of the crystal structures of membrane proteins via iodide–single-wavelength anomalous diffraction (I-SAD). The potential universality of the method is based on a common feature of membrane proteins—the availability at the hydrophobic-hydrophilic interface of positively charged amino acid residues with which iodide strongly interacts. We demonstrate the solution using I-SAD of four crystal structures representing different classes of membrane proteins, including a human G protein–coupled receptor (GPCR), and we show that I-SAD can be applied using data collection strategies based on either standard or serial x-ray crystallography techniques.
LB  - PUB:(DE-HGF)16
C6  - pmid:28508075
UR  - <Go to ISI:>//WOS:000401955300041
DO  - DOI:10.1126/sciadv.1602952
UR  - https://juser.fz-juelich.de/record/834108
ER  -