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@ARTICLE{Melnikov:834108,
author = {Melnikov, Igor and Polovinkin, Vitaly and Kovalev, Kirill
and Gushchin, Ivan and Shevtsov, Mikhail and Shevchenko,
Vitaly and Mishin, Alexey and Alekseev, Alexey and
Rodriguez-Valera, Francisco and Borshchevskiy, Valentin and
Cherezov, Vadim and Leonard, Gordon A. and Gordeliy,
Valentin and Popov, Alexander},
title = {{F}ast iodide-{SAD} phasing for high-throughput membrane
protein structure determination},
journal = {Science advances},
volume = {3},
number = {5},
issn = {2375-2548},
address = {Washington, DC [u.a.]},
publisher = {Assoc.},
reportid = {FZJ-2017-04109},
pages = {e1602952},
year = {2017},
abstract = {We describe a fast, easy, and potentially universal method
for the de novo solution of the crystal structures of
membrane proteins via iodide–single-wavelength anomalous
diffraction (I-SAD). The potential universality of the
method is based on a common feature of membrane
proteins—the availability at the hydrophobic-hydrophilic
interface of positively charged amino acid residues with
which iodide strongly interacts. We demonstrate the solution
using I-SAD of four crystal structures representing
different classes of membrane proteins, including a human G
protein–coupled receptor (GPCR), and we show that I-SAD
can be applied using data collection strategies based on
either standard or serial x-ray crystallography techniques.},
cin = {ICS-6},
ddc = {500},
cid = {I:(DE-Juel1)ICS-6-20110106},
pnm = {551 - Functional Macromolecules and Complexes (POF3-551)},
pid = {G:(DE-HGF)POF3-551},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:28508075},
UT = {WOS:000401955300041},
doi = {10.1126/sciadv.1602952},
url = {https://juser.fz-juelich.de/record/834108},
}