Hauptseite > Publikationsdatenbank > Fast iodide-SAD phasing for high-throughput membrane protein structure determination > print

001     834108
005     20210129230541.0
024 7 _ |a 10.1126/sciadv.1602952
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100 1 _ |0 P:(DE-HGF)0
|a Melnikov, Igor
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245 _ _ |a Fast iodide-SAD phasing for high-throughput membrane protein structure determination
260 _ _ |a Washington, DC [u.a.]
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520 _ _ |a We describe a fast, easy, and potentially universal method for the de novo solution of the crystal structures of membrane proteins via iodide–single-wavelength anomalous diffraction (I-SAD). The potential universality of the method is based on a common feature of membrane proteins—the availability at the hydrophobic-hydrophilic interface of positively charged amino acid residues with which iodide strongly interacts. We demonstrate the solution using I-SAD of four crystal structures representing different classes of membrane proteins, including a human G protein–coupled receptor (GPCR), and we show that I-SAD can be applied using data collection strategies based on either standard or serial x-ray crystallography techniques.
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|a Polovinkin, Vitaly
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|a Kovalev, Kirill
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700 1 _ |0 P:(DE-Juel1)165798
|a Gushchin, Ivan
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700 1 _ |0 P:(DE-HGF)0
|a Shevtsov, Mikhail
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700 1 _ |0 P:(DE-Juel1)171538
|a Shevchenko, Vitaly
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|a Mishin, Alexey
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700 1 _ |0 P:(DE-Juel1)165628
|a Alekseev, Alexey
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700 1 _ |0 P:(DE-HGF)0
|a Rodriguez-Valera, Francisco
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700 1 _ |0 P:(DE-Juel1)144613
|a Borshchevskiy, Valentin
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700 1 _ |0 P:(DE-HGF)0
|a Cherezov, Vadim
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700 1 _ |0 P:(DE-HGF)0
|a Leonard, Gordon A.
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700 1 _ |0 P:(DE-Juel1)131964
|a Gordeliy, Valentin
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|a Popov, Alexander
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