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@ARTICLE{Akers:834153,
      author       = {Akers, Peter W. and Dingley, Andrew and Swift, Simon and
                      Nelson, Andrew R. J. and Martin, Julie and McGillivray,
                      Duncan J.},
      title        = {{U}sing {N}eutron {R}eflectometry to {C}haracterize
                      {A}ntimicrobial {P}rotein {S}urface {C}oatings},
      journal      = {The journal of physical chemistry / B},
      volume       = {121},
      number       = {24},
      issn         = {1089-5647},
      address      = {Washington, DC},
      publisher    = {Soc.},
      reportid     = {FZJ-2017-04151},
      pages        = {5908–5916},
      year         = {2017},
      abstract     = {Understanding the interaction of adsorbed or covalently
                      immobilized proteins with solid substrates at the molecular
                      level guides the successful design of functionalized
                      surfaces used in biomedical applications. In this report,
                      neutron reflectometry (NR) was used to characterize the
                      structure of surface-attached antimicrobial protein films,
                      with antimicrobial activity assessed using an adaption of
                      the Japanese Industrial Standard Test JIS Z 2801. NR allowed
                      parameters influencing bioactivity to be measured at
                      nanometer resolution and for conclusions about structural
                      characteristics relating to bioactivity to be drawn.
                      Hydramacin-1 (HM-1) and lysozyme were covalently attached to
                      poly(methyl methacrylate) (PMMA) and
                      3-aminopropyltriethoxysilane (APTES) films in the presence
                      and absence of a four-unit poly(ethylene glycol) PEG-based
                      spacer and measured using NR, followed by antimicrobial
                      assays. APTES-PEG-protein films were structurally unique,
                      with a layer of $80\%$ water directly beneath the protein
                      layer, and were the only films that displayed antimicrobial
                      activity against Escherichia coli and Bacillus subtilis. The
                      hydration content of these films combined with the subtle
                      difference in the PEG layer thickness of APTES versus PMMA
                      films played a role in defining antimicrobial activity of
                      the prepared surface coatings.},
      cin          = {ICS-6},
      ddc          = {530},
      cid          = {I:(DE-Juel1)ICS-6-20110106},
      pnm          = {553 - Physical Basis of Diseases (POF3-553)},
      pid          = {G:(DE-HGF)POF3-553},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000404202000003},
      doi          = {10.1021/acs.jpcb.7b02886},
      url          = {https://juser.fz-juelich.de/record/834153},
}