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@ARTICLE{Gro:835094,
author = {Groß, Felicitas and Rudolf, Eva-Esther and Thiele, Björn
and Durner, Jörg and Astier, Jeremy},
title = {{C}opper amine oxidase 8 regulates arginine-dependent
nitric oxide production in {A}rabidopsis thaliana},
journal = {The journal of experimental botany},
volume = {68},
number = {9},
issn = {1460-2431},
address = {Oxford},
publisher = {Oxford Univ. Press},
reportid = {FZJ-2017-04962},
pages = {2149 - 2162},
year = {2017},
abstract = {Nitric oxide (NO) is a key signaling molecule in plants,
regulating a wide range of physiological processes. However,
its origin in plants remains unclear. It can be generated
from nitrite through a reductive pathway, notably via the
action of the nitrate reductase (NR), and evidence suggests
an additional oxidative pathway, involving arginine. From an
initial screen of potential Arabidopsis thaliana mutants
impaired in NO production, we identified copper amine
oxidase 8 (CuAO8). Two cuao8 mutant lines displayed a
decreased NO production in seedlings after elicitor
treatment and salt stress. The NR-dependent pathway was not
responsible for the impaired NO production as no change in
NR activity was found in the mutants. However, total
arginase activity was strongly increased in cuao8 knockout
mutants after salt stress. Moreover, NO production could be
restored in the mutants by arginase inhibition or arginine
addition. Furthermore, arginine supplementation reversed the
root growth phenotype observed in the mutants. These results
demonstrate that CuAO8 participates in NO production by
influencing arginine availability through the modulation of
arginase activity. The influence of CuAO8 on
arginine-dependent NO synthesis suggests a new regulatory
pathway for NO production in plants.},
cin = {IBG-2},
ddc = {580},
cid = {I:(DE-Juel1)IBG-2-20101118},
pnm = {582 - Plant Science (POF3-582)},
pid = {G:(DE-HGF)POF3-582},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000402272700008},
pubmed = {pmid:28383668},
doi = {10.1093/jxb/erx105},
url = {https://juser.fz-juelich.de/record/835094},
}