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@ARTICLE{Boeske:836457,
      author       = {Boeske, Alexandra and Schwarten, Melanie and ma, peixiang
                      and Tusche, Markus and Mötter, Jessica and Neudecker,
                      Philipp and Möller, Christina and Hoffmann, Silke and
                      Willbold, Dieter},
      title        = {{D}irect binding to {GABARAP} family members is essential
                      for {HIV}-1 {N}ef plasma membrane localization},
      journal      = {Scientific reports},
      volume       = {7},
      issn         = {2045-2322},
      address      = {London},
      publisher    = {Nature Publishing Group},
      reportid     = {FZJ-2017-05575},
      pages        = {5979},
      year         = {2017},
      abstract     = {HIV-1 Nef is an important pathogenic factor for HIV/AIDS
                      pathogenesis. Studies have shown that the association of Nef
                      with the inner leaflet of the plasma membrane and with
                      endocytic and perinuclear vesicles is essential for most
                      activities of Nef. Using purified recombinant proteins in
                      pull-down assays and by co-immunoprecipitation assays we
                      demonstrate that Nef binds directly and specifically to all
                      GABARAP family members, but not to LC3 family members. Based
                      on nuclear magnetic resonance (NMR) experiments we showed
                      that Nef binds to GABARAP via two surface exposed
                      hydrophobic pockets. S53 and F62 of GABARAP were identified
                      as key residues for the interaction with Nef. During
                      live-cell fluorescence microscopy an accumulation of Nef and
                      all GABARAP family members in vesicular structures
                      throughout the cytoplasm and at the plasma membrane was
                      observed. This plasma membrane accumulation was
                      significantly reduced after knocking down GABARAP, GABARAPL1
                      and GABARAPL2 with respective siRNAs. We identified GABARAPs
                      as the first known direct interaction partners of Nef that
                      are essential for its plasma membrane localization.},
      cin          = {ICS-6},
      ddc          = {000},
      cid          = {I:(DE-Juel1)ICS-6-20110106},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000405907800033},
      pubmed       = {pmid:28729737},
      doi          = {10.1038/s41598-017-06319-4},
      url          = {https://juser.fz-juelich.de/record/836457},
}