TY  - JOUR
AU  - Marx, Manuel
AU  - Qi, Guanxiao
AU  - Hanganu-Opatz, Ileana L.
AU  - Kilb, Werner
AU  - Luhmann, Heiko J.
AU  - Feldmeyer, Dirk
TI  - Neocortical Layer 6B as a Remnant of the Subplate - A Morphological Comparison
JO  - Cerebral cortex
VL  - 27
IS  - 2
SN  - 1460-2199
CY  - Oxford
PB  - Oxford Univ. Press
M1  - FZJ-2017-05660
SP  - 1011-1026
PY  - 2017
AB  - The fate of the subplate (SP) is still a matter of debate. The SP and layer 6 (which is ontogenetically the oldest and innermostneocortical lamina) develop coincidentally. Yet, the function of sublamina 6B is largely unknown. It has been suggested that itconsists partly of neurons from the transient SP, however, experimental evidence for this hypothesis is still missing. To obtainfirst insights into the neuronal complement of layer 6B in the somatosensory rat barrel cortex, we used biocytin stainings of SPneurons (aged 0–4 postnatal days, PND) and layer 6B neurons (PND 11–35) obtained during in vitro whole-cell patch-clamprecordings. Neurons were reconstructed for a quantitative characterization of their axonal and dendritic morphology. An unsupervised cluster analysis revealed that the SP and layer 6B consist of heterogeneous but comparable neuronal cell populations. Both contain 5 distinct spine-bearing cell types whose relative fractions change with increasing age. Pyramidal cells were more prominent in layer 6B, whereas non-pyramidal neurons were less frequent. Because of the high morphological similarity of SP and layer 6B neurons, we suggest that layer 6B consists of persistent non-pyramidal neurons from the SP and cortical L6B pyramidal neurons.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000397257600010
C6  - pmid:26637449
DO  - DOI:10.1093/cercor/bhv279
UR  - https://juser.fz-juelich.de/record/836585
ER  -