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@ARTICLE{BrkerLai:836931,
author = {Bröker‐Lai, Jenny and Kollewe, Astrid and
Schindeldecker, Barbara and Pohle, Jörg and Nguyen Chi,
Vivan and Mathar, Ilka and Guzman, Raul and Schwarz, Yvonne
and Lai, Alan and Weißgerber, Petra and Schwegler, Herbert
and Dietrich, Alexander and Both, Martin and Sprengel, Rolf
and Draguhn, Andreas and Köhr, Georg and Fakler, Bernd and
Flockerzi, Veit and Bruns, Dieter and Freichel, Marc},
title = {{H}eteromeric channels formed by {TRPC}1, {TRPC}4 and
{TRPC}5 define hippocampal synaptic transmission and working
memory},
journal = {The EMBO journal},
volume = {36},
issn = {1460-2075},
address = {Heidelberg},
publisher = {EMBO Press},
reportid = {FZJ-2017-05957},
pages = {e201696369 -},
year = {2017},
abstract = {Canonical transient receptor potential (TRPC) channels
influence various neuronal functions. Using quantitative
high‐resolution mass spectrometry, we demonstrate that
TRPC1, TRPC4, and TRPC5 assemble into heteromultimers with
each other, but not with other TRP family members in the
mouse brain and hippocampus. In hippocampal neurons from
Trpc1/Trpc4/Trpc5‐triple‐knockout (Trpc1/4/5−/−)
mice, lacking any TRPC1‐, TRPC4‐, or TRPC5‐containing
channels, action potential‐triggered excitatory
postsynaptic currents (EPSCs) were significantly reduced,
whereas frequency, amplitude, and kinetics of quantal
miniature EPSC signaling remained unchanged. Likewise,
evoked postsynaptic responses in hippocampal slice
recordings and transient potentiation after tetanic
stimulation were decreased. In vivo, Trpc1/4/5−/− mice
displayed impaired cross‐frequency coupling in hippocampal
networks and deficits in spatial working memory, while
spatial reference memory was unaltered. Trpc1/4/5−/−
animals also exhibited deficiencies in adapting to a new
challenge in a relearning task. Our results indicate the
contribution of heteromultimeric channels from TRPC1, TRPC4,
and TRPC5 subunits to the regulation of mechanisms
underlying spatial working memory and flexible relearning by
facilitating proper synaptic transmission in hippocampal
neurons.},
cin = {ICS-4},
ddc = {570},
cid = {I:(DE-Juel1)ICS-4-20110106},
pnm = {552 - Engineering Cell Function (POF3-552)},
pid = {G:(DE-HGF)POF3-552},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000410763900011},
pubmed = {pmid:28790178},
doi = {10.15252/embj.201696369},
url = {https://juser.fz-juelich.de/record/836931},
}