000836989 001__ 836989
000836989 005__ 20210129231143.0
000836989 0247_ $$2doi$$a10.1016/j.cbd.2017.07.004
000836989 0247_ $$2ISSN$$a1744-117X
000836989 0247_ $$2ISSN$$a1878-0407
000836989 0247_ $$2pmid$$apmid:28806688
000836989 0247_ $$2WOS$$aWOS:000414815200004
000836989 0247_ $$2altmetric$$aaltmetric:23846027
000836989 037__ $$aFZJ-2017-06011
000836989 041__ $$aEnglish
000836989 082__ $$a540
000836989 1001_ $$0P:(DE-HGF)0$$aSonnack, Laura$$b0$$eCorresponding author
000836989 245__ $$aConcentration dependent transcriptome responses of zebrafish embryos after exposure to cadmium, cobalt and copper
000836989 260__ $$aNew York, NY [u.a.]$$bElsevier$$c2017
000836989 3367_ $$2DRIVER$$aarticle
000836989 3367_ $$2DataCite$$aOutput Types/Journal article
000836989 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1506325972_17755
000836989 3367_ $$2BibTeX$$aARTICLE
000836989 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000836989 3367_ $$00$$2EndNote$$aJournal Article
000836989 520__ $$aEnvironmental metals are known to cause harmful effects to fish of which many molecular mechanisms still require elucidation. Particularly concentration dependence of gene expression effects is unclear. Focusing on this matter, zebrafish embryo toxicity tests were used in combination with transcriptomics. Embryos were exposed to three concentrations of copper (CuSO4), cadmium (CdCl2) and cobalt (CoSO4) from just after fertilization until the end of the 48hpf pre- and 96hpf post-hatch stage. The RNA was then analyzed on Agilent's Zebrafish (V3, 4×44K) arrays. Enrichment for GO terms of biological processes illustrated for cadmium that most affected GO terms were represented in all three concentrations, while for cobalt and copper most GO terms were represented in the lowest test concentration only. This suggested a different response to the non-essential cadmium than cobalt and copper. In cobalt and copper treated embryos, many developmental and cellular processes as well as the Wnt and Notch signaling pathways, were found significantly enriched. Also, different exposure concentrations affected varied functional networks. In contrast, the largest clusters of enriched GO terms for all three concentrations of cadmium included responses to cadmium ion, metal ion, xenobiotic stimulus, stress and chemicals. However, concentration dependence of mRNA levels was evident for several genes in all metal exposures. Some of these genes may be indicative of the mechanisms of action of the individual metals in zebrafish embryos. Real-time quantitative RT-PCR (qRT-PCR) verified the microarray data for mmp9, mt2, cldnb and nkx2.2a.
000836989 536__ $$0G:(DE-HGF)POF3-899$$a899 - ohne Topic (POF3-899)$$cPOF3-899$$fPOF III$$x0
000836989 588__ $$aDataset connected to CrossRef
000836989 65027 $$0V:(DE-MLZ)SciArea-160$$2V:(DE-HGF)$$aBiology$$x0
000836989 7001_ $$0P:(DE-HGF)0$$aKlawonn, Thorsten$$b1
000836989 7001_ $$0P:(DE-Juel1)133469$$aKriehuber, Ralf$$b2$$ufzj
000836989 7001_ $$0P:(DE-HGF)0$$aHollert, Henner$$b3
000836989 7001_ $$0P:(DE-HGF)0$$aSchäfers, Christoph$$b4
000836989 7001_ $$0P:(DE-HGF)0$$aFenske, Martina$$b5$$eCorresponding author
000836989 773__ $$0PERI:(DE-600)2224862-6$$a10.1016/j.cbd.2017.07.004$$gVol. 24, p. 29 - 40$$p29 - 40$$tComparative biochemistry and physiology / D$$v24$$x1744-117X$$y2017
000836989 8564_ $$uhttps://juser.fz-juelich.de/record/836989/files/1-s2.0-S1744117X17300588-main.pdf$$yRestricted
000836989 8564_ $$uhttps://juser.fz-juelich.de/record/836989/files/1-s2.0-S1744117X17300588-main.gif?subformat=icon$$xicon$$yRestricted
000836989 8564_ $$uhttps://juser.fz-juelich.de/record/836989/files/1-s2.0-S1744117X17300588-main.jpg?subformat=icon-1440$$xicon-1440$$yRestricted
000836989 8564_ $$uhttps://juser.fz-juelich.de/record/836989/files/1-s2.0-S1744117X17300588-main.jpg?subformat=icon-180$$xicon-180$$yRestricted
000836989 8564_ $$uhttps://juser.fz-juelich.de/record/836989/files/1-s2.0-S1744117X17300588-main.jpg?subformat=icon-640$$xicon-640$$yRestricted
000836989 8564_ $$uhttps://juser.fz-juelich.de/record/836989/files/1-s2.0-S1744117X17300588-main.pdf?subformat=pdfa$$xpdfa$$yRestricted
000836989 909CO $$ooai:juser.fz-juelich.de:836989$$pVDB
000836989 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)133469$$aForschungszentrum Jülich$$b2$$kFZJ
000836989 9131_ $$0G:(DE-HGF)POF3-899$$1G:(DE-HGF)POF3-890$$2G:(DE-HGF)POF3-800$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bProgrammungebundene Forschung$$lohne Programm$$vohne Topic$$x0
000836989 9141_ $$y2017
000836989 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline
000836989 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database
000836989 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bCOMP BIOCHEM PHYS D : 2015
000836989 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS
000836989 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bThomson Reuters Master Journal List
000836989 915__ $$0StatID:(DE-HGF)0110$$2StatID$$aWoS$$bScience Citation Index
000836989 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection
000836989 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded
000836989 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences
000836989 915__ $$0StatID:(DE-HGF)1040$$2StatID$$aDBCoverage$$bZoological Record
000836989 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews
000836989 915__ $$0StatID:(DE-HGF)9900$$2StatID$$aIF < 5
000836989 920__ $$lyes
000836989 9201_ $$0I:(DE-Juel1)S-US-20090406$$kS-US$$lSicherheit und Strahlenschutz, Umgebungsüberwachung,Strahlenbiologie$$x0
000836989 980__ $$ajournal
000836989 980__ $$aVDB
000836989 980__ $$aI:(DE-Juel1)S-US-20090406
000836989 980__ $$aUNRESTRICTED