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@ARTICLE{Sonnack:836989,
      author       = {Sonnack, Laura and Klawonn, Thorsten and Kriehuber, Ralf
                      and Hollert, Henner and Schäfers, Christoph and Fenske,
                      Martina},
      title        = {{C}oncentration dependent transcriptome responses of
                      zebrafish embryos after exposure to cadmium, cobalt and
                      copper},
      journal      = {Comparative biochemistry and physiology / D},
      volume       = {24},
      issn         = {1744-117X},
      address      = {New York, NY [u.a.]},
      publisher    = {Elsevier},
      reportid     = {FZJ-2017-06011},
      pages        = {29 - 40},
      year         = {2017},
      abstract     = {Environmental metals are known to cause harmful effects to
                      fish of which many molecular mechanisms still require
                      elucidation. Particularly concentration dependence of gene
                      expression effects is unclear. Focusing on this matter,
                      zebrafish embryo toxicity tests were used in combination
                      with transcriptomics. Embryos were exposed to three
                      concentrations of copper (CuSO4), cadmium (CdCl2) and cobalt
                      (CoSO4) from just after fertilization until the end of the
                      48hpf pre- and 96hpf post-hatch stage. The RNA was then
                      analyzed on Agilent's Zebrafish (V3, 4×44K) arrays.
                      Enrichment for GO terms of biological processes illustrated
                      for cadmium that most affected GO terms were represented in
                      all three concentrations, while for cobalt and copper most
                      GO terms were represented in the lowest test concentration
                      only. This suggested a different response to the
                      non-essential cadmium than cobalt and copper. In cobalt and
                      copper treated embryos, many developmental and cellular
                      processes as well as the Wnt and Notch signaling pathways,
                      were found significantly enriched. Also, different exposure
                      concentrations affected varied functional networks. In
                      contrast, the largest clusters of enriched GO terms for all
                      three concentrations of cadmium included responses to
                      cadmium ion, metal ion, xenobiotic stimulus, stress and
                      chemicals. However, concentration dependence of mRNA levels
                      was evident for several genes in all metal exposures. Some
                      of these genes may be indicative of the mechanisms of action
                      of the individual metals in zebrafish embryos. Real-time
                      quantitative RT-PCR (qRT-PCR) verified the microarray data
                      for mmp9, mt2, cldnb and nkx2.2a.},
      cin          = {S-US},
      ddc          = {540},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:28806688},
      UT           = {WOS:000414815200004},
      doi          = {10.1016/j.cbd.2017.07.004},
      url          = {https://juser.fz-juelich.de/record/836989},
}