TY  - JOUR
AU  - Baumgart, Meike
AU  - Unthan, Simon
AU  - Kloss, Ramona
AU  - Radek, Andreas
AU  - Polen, Tino
AU  - Tenhaef, Niklas
AU  - Müller, Moritz Fabian
AU  - Küberl, Andreas
AU  - Siebert, Daniel
AU  - Brühl, Natalie
AU  - Marin, Kay
AU  - Hans, Stephan
AU  - Krämer, Reinhard
AU  - Bott, Michael
AU  - Kalinowski, Joern
AU  - Wiechert, Wolfgang
AU  - Seibold, Gerd
AU  - Frunzke, Julia
AU  - Wendisch, Volker
AU  - Noack, Stephan
AU  - Rückert, Christian
TI  - Corynebacterium glutamicum chassis C1*: Building and testing a novel platform host for synthetic biology and industrial biotechnology
JO  - ACS synthetic biology
VL  - 7
IS  - 1
SN  - 2161-5063
CY  - Washington, DC
PB  - ACS
M1  - FZJ-2017-06062
SP  - 132–144
PY  - 2018
N1  - Biotechnologie 1
AB  - Targeted top-down strategies for genome reduction are considered to have a high potential for providing robust basic strains for synthetic biology and industrial biotechnology. Recently, we created a library of 26 genome-reduced strains of Corynebacterium glutamicum carrying broad deletions in single gene clusters and showing wild-type-like biological fitness. Here, we proceeded with combinatorial deletions of these irrelevant gene clusters in two parallel orders, and the resulting library of 28 strains was characterized under various environmental conditions. The final chassis strain C1* carries a genome reduction of 13.4% (412 deleted genes) and shows wild-type-like growth behavior in defined medium with d-glucose as carbon and energy source. Moreover, C1* proves to be robust against several stresses (including oxygen limitation) and shows long-term growth stability under defined and complex medium conditions. In addition to providing a novel prokaryotic chassis strain, our results comprise a large strain library and a revised genome annotation list, which will be valuable sources for future systemic studies of C. glutamicum.
LB  - PUB:(DE-HGF)16
C6  - pmid:28803482
UR  - <Go to ISI:>//WOS:000423252800015
DO  - DOI:10.1021/acssynbio.7b00261
UR  - https://juser.fz-juelich.de/record/837053
ER  -