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@ARTICLE{Baumgart:837053,
      author       = {Baumgart, Meike and Unthan, Simon and Kloss, Ramona and
                      Radek, Andreas and Polen, Tino and Tenhaef, Niklas and
                      Müller, Moritz Fabian and Küberl, Andreas and Siebert,
                      Daniel and Brühl, Natalie and Marin, Kay and Hans, Stephan
                      and Krämer, Reinhard and Bott, Michael and Kalinowski,
                      Joern and Wiechert, Wolfgang and Seibold, Gerd and Frunzke,
                      Julia and Wendisch, Volker and Noack, Stephan and Rückert,
                      Christian},
      title        = {{C}orynebacterium glutamicum chassis {C}1*: {B}uilding and
                      testing a novel platform host for synthetic biology and
                      industrial biotechnology},
      journal      = {ACS synthetic biology},
      volume       = {7},
      number       = {1},
      issn         = {2161-5063},
      address      = {Washington, DC},
      publisher    = {ACS},
      reportid     = {FZJ-2017-06062},
      pages        = {132–144},
      year         = {2018},
      note         = {Biotechnologie 1},
      abstract     = {Targeted top-down strategies for genome reduction are
                      considered to have a high potential for providing robust
                      basic strains for synthetic biology and industrial
                      biotechnology. Recently, we created a library of 26
                      genome-reduced strains of Corynebacterium glutamicum
                      carrying broad deletions in single gene clusters and showing
                      wild-type-like biological fitness. Here, we proceeded with
                      combinatorial deletions of these irrelevant gene clusters in
                      two parallel orders, and the resulting library of 28 strains
                      was characterized under various environmental conditions.
                      The final chassis strain C1* carries a genome reduction of
                      $13.4\%$ (412 deleted genes) and shows wild-type-like growth
                      behavior in defined medium with d-glucose as carbon and
                      energy source. Moreover, C1* proves to be robust against
                      several stresses (including oxygen limitation) and shows
                      long-term growth stability under defined and complex medium
                      conditions. In addition to providing a novel prokaryotic
                      chassis strain, our results comprise a large strain library
                      and a revised genome annotation list, which will be valuable
                      sources for future systemic studies of C. glutamicum.},
      cin          = {IBG-1},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {581 - Biotechnology (POF3-581)},
      pid          = {G:(DE-HGF)POF3-581},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:28803482},
      UT           = {WOS:000423252800015},
      doi          = {10.1021/acssynbio.7b00261},
      url          = {https://juser.fz-juelich.de/record/837053},
}