TY  - JOUR
AU  - Figueroa-Miranda, Gabriela
AU  - Feng, Lingyan
AU  - Shiu, Simon Chi-Chin
AU  - Dirkzwager, Roderick Marshall
AU  - Cheung, Yee-Wai
AU  - Tanner, Julian Alexander
AU  - Schöning, Michael Josef
AU  - Offenhäusser, Andreas
AU  - Mayer, Dirk
TI  - Aptamer-based electrochemical biosensor for highly sensitive and selective malaria detection with adjustable dynamic response range and reusability
JO  - Sensors and actuators  / B
VL  - 255
SN  - 0925-4005
CY  - Amsterdam [u.a.]
PB  - Elsevier Science
M1  - FZJ-2017-06112
SP  - 235 - 243
PY  - 2018
AB  - Malaria infection remains a significant risk for much of the population of tropical and subtropical areas, particularly in developing countries. Therefore, it is of high importance to develop sensitive, accurate and inexpensive malaria diagnosis tests. Here, we present a novel aptamer-based electrochemical biosensor (aptasensor) for malaria detection by impedance spectroscopy, through the specific recognition between a highly discriminatory DNA aptamer and its target Plasmodium falciparum lactate dehydrogenase (PfLDH). Interestingly, due to the isoelectric point (pI) of PfLDH, the aptasensor response showed an adjustable detection range based on the different protein net-charge at variable pH environments. The specific aptamer recognition allows sensitive protein detection with an expanded detection range and a low detection limit, as well as a high specificity for PfLDH compared to analogous proteins. The specific feasibility of the aptasensor is further demonstrated by detection of the target PfLDH in human serum. Furthermore, the aptasensor can be easily regenerated and thus applied for multiple usages. The robustness, sensitivity, and reusability of the presented aptasensor make it a promising candidate for point-of-care diagnostic systems.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000414151800028
DO  - DOI:10.1016/j.snb.2017.07.117
UR  - https://juser.fz-juelich.de/record/837123
ER  -