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@PHDTHESIS{Mtter:837194,
      author       = {Mötter, Jessica},
      title        = {{I}dentifizierung von {I}nteraktionspartnern für {HIV}-1
                      {N}ef und ihre potentielle {R}elevanz bei der {E}ntwicklung
                      der {HIV}-assoziierten {D}emenz},
      volume       = {19},
      school       = {Universität Düsseldorf},
      type         = {Dr.},
      address      = {Jülich},
      publisher    = {Forschungszentrum Jülich GmbH Zentralbibliothek, Verlag},
      reportid     = {FZJ-2017-06173},
      isbn         = {978-3-89336-604-0},
      series       = {Schriften des Forschungszentrums Jülich. Reihe Gesundheit
                      / Health},
      pages        = {VI, 173 S : Ill., graph. Darst},
      year         = {2010},
      note         = {Keine Online-Freischaltung; Universität Düsseldorf,
                      Diss., 2010},
      abstract     = {Neuro-Aids as a consequence of infection with human
                      immundeficiency virus (HIV), becomes increasingly important
                      due to considerably more efficient anti-AIDS therapies that
                      result in longer life expectancy. Besides HIV Tat the viral
                      Nef protein is hypothesized to play a role in development of
                      Neuro-AIDS. Till today the detailed function of HIV-1 Nef
                      protein in the development of neurological manifestations of
                      an HIV infection is not known. Possibly, Nef could
                      contribute to open the blood-brain barrier by activating
                      matrix metalloproteinase-9 allowing HIV to enter the central
                      nervous system. Furthermore, Nef is known to induce
                      chemokine CCL2/MCP1 expression, which causes infiltration of
                      the brain by monocytes. It is also known that
                      oligodendrocytes initiate vacuolar myelopathy. This is
                      amongst others a disease pattern of HIV associated
                      neuromanifestations. Thus, for elucidation of HIV associated
                      neuromanifestations on the molecular level Nef is an
                      interesting candidate. Aim of the present work was the
                      identification of new cellular interaction partners for
                      HIV-1 Nef protein with a potential role in development of
                      HIV associated dementia (HAD). Knowledge of new brain
                      specific interaction partners of Nef may lead to a better
                      understanding of Nef cellular functions. Use of a modern
                      yeast two-hybrid system yielded succeessful identification
                      of proteins as so far unknown interaction partners of
                      membrane associated Nef. A total of 21 proteins, including
                      17 membrane proteins, were verified as interaction partners
                      of Nef in the yeast system. Some of them may be relevant for
                      HIV/HAD. B-cell receptor-associated protein 31 (Bcap31) was
                      the second most prevalent hit. The interaction of Nef with
                      Bcap31 could be confirmed with coimmunprecipitation in yeast
                      cells. Furthermore, colocalization in Neuro2A cells in
                      structures at plasma membrane succeeded. The glycoprotein
                      M6B (GPM6B) was the most dominant hit in the screen and its
                      interaction with Nef was characterized in detail in the
                      present work. The interaction of Nef with GPM6B could be
                      verified by coimmunprecipitation from yeast cell extract and
                      pulldown assays using rat brain. Furthermore, Nef and GPM6B
                      colocalized in Neuro2A cells and the relevant binding region
                      in GPM6B could be defined to a 16 amino acid residue region,
                      which is very similar to the Nef-binding region of human
                      CD4. Fluorescence measurements resulted in a submicromolar
                      dissociation constant for this interaction. The results
                      obtained in the present work deliver important contributions
                      for a deeper understanding of Nef’s function in HAD.},
      keywords     = {HIV (gnd) / Gen nef (gnd) / Demenz (gnd)},
      cin          = {ISB-3},
      ddc          = {610},
      cid          = {I:(DE-Juel1)VDB942},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)3 / PUB:(DE-HGF)11},
      url          = {https://juser.fz-juelich.de/record/837194},
}