TY  - JOUR
AU  - Toulmin, A.
AU  - Baltierra-Jasso, L. E.
AU  - Morten, M. J.
AU  - Sabir, T.
AU  - McGlynn, P.
AU  - Schröder, Gunnar
AU  - Smith, B. O.
AU  - Magennis, S. W.
TI  - Conformational Heterogeneity in a Fully Complementary DNA Three-Way Junction with a GC-Rich Branchpoint.
JO  - Biochemistry
VL  - 56
IS  - 37
SN  - 0006-2960
CY  - Columbus, Ohio
PB  - American Chemical Society
M1  - FZJ-2017-06586
SP  - 4985–4991
PY  - 2017
AB  - DNA three-way junctions (3WJs) are branched structures that serve as important biological intermediates and as components in DNA nanostructures. We recently derived the global structure of a fully complementary 3WJ and found that it contained unpaired bases at the branchpoint, which is consistent with previous observations of branch flexibility and branchpoint reactivity. By combining high-resolution single-molecule Förster resonance energy transfer, molecular modeling, time-resolved ensemble fluorescence spectroscopy, and the first 19F nuclear magnetic resonance observations of fully complementary 3WJs, we now show that the 3WJ structure can adopt multiple distinct conformations depending upon the sequence at the branchpoint. A 3WJ with a GC-rich branchpoint adopts an open conformation with unpaired bases at the branch and at least one additional conformation with an increased number of base interactions at the branchpoint. This structural diversity has implications for branch interactions and processing in vivo and for technological applications.
LB  - PUB:(DE-HGF)16
C6  - pmid:28820590
UR  - <Go to ISI:>//WOS:000411548900009
DO  - DOI:10.1021/acs.biochem.7b00677
UR  - https://juser.fz-juelich.de/record/837797
ER  -