%0 Journal Article
%A Su, Shijie
%A Hou, Zhaofeng
%A Liu, Dandan
%A Jia, Chun-Lin
%A Wang, Lele
%A Xu, Jinjun
%A Tao, Jianping
%T Comparative transcriptome analysis of second- and third-generation merozoites of Eimeria necatrix
%J Parasites & vectors
%V 10
%N 1
%@ 1756-3305
%C London
%I BioMed Central
%M FZJ-2017-06691
%P 388
%D 2017
%X BackgroundEimeria is a common genus of apicomplexan parasites that infect diverse vertebrates, most notably poultry, causing serious disease and economic losses. Eimeria species have complex life-cycles consisting of three developmental stages. However, the molecular basis of the Eimeria reproductive mode switch remains an enigma.MethodsTotal RNA extracted from second- (MZ-2) and third-generation merozoites (MZ-3) of Eimeria necatrix was subjected to transcriptome analysis using RNA sequencing (RNA-seq) followed by qRT-PCR validation.ResultsA total of 6977 and 6901 unigenes were obtained from MZ-2 and MZ-3, respectively. Approximately 2053 genes were differentially expressed genes (DEGs) between MZ-2 and MZ-3. Compared with MZ-2, 837 genes were upregulated and 1216 genes were downregulated in MZ-3. Approximately 95 genes in MZ-2 and 48 genes in MZ-3 were further identified to have stage-specific expression. Gene ontology category and KEGG analysis suggested that 216 upregulated genes in MZ-2 were annotated by 70 GO assignments, 242 upregulated genes were associated with 188 signal pathways, while 321 upregulated genes in MZ-3 were annotated by 56 GO assignments, 322 upregulated genes were associated with 168 signal pathways. The molecular functions of upregulated genes in MZ-2 were mainly enriched for protein degradation and amino acid metabolism. The molecular functions of upregulated genes in MZ-3 were mainly enriched for transcriptional activity, cell proliferation and cell differentiation.
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000408039700002
%$ pmid:28814335
%R 10.1186/s13071-017-2325-z
%U https://juser.fz-juelich.de/record/837921