TY  - JOUR
AU  - Su, Shijie
AU  - Hou, Zhaofeng
AU  - Liu, Dandan
AU  - Jia, Chun-Lin
AU  - Wang, Lele
AU  - Xu, Jinjun
AU  - Tao, Jianping
TI  - Comparative transcriptome analysis of second- and third-generation merozoites of Eimeria necatrix
JO  - Parasites & vectors
VL  - 10
IS  - 1
SN  - 1756-3305
CY  - London
PB  - BioMed Central
M1  - FZJ-2017-06691
SP  - 388
PY  - 2017
AB  - BackgroundEimeria is a common genus of apicomplexan parasites that infect diverse vertebrates, most notably poultry, causing serious disease and economic losses. Eimeria species have complex life-cycles consisting of three developmental stages. However, the molecular basis of the Eimeria reproductive mode switch remains an enigma.MethodsTotal RNA extracted from second- (MZ-2) and third-generation merozoites (MZ-3) of Eimeria necatrix was subjected to transcriptome analysis using RNA sequencing (RNA-seq) followed by qRT-PCR validation.ResultsA total of 6977 and 6901 unigenes were obtained from MZ-2 and MZ-3, respectively. Approximately 2053 genes were differentially expressed genes (DEGs) between MZ-2 and MZ-3. Compared with MZ-2, 837 genes were upregulated and 1216 genes were downregulated in MZ-3. Approximately 95 genes in MZ-2 and 48 genes in MZ-3 were further identified to have stage-specific expression. Gene ontology category and KEGG analysis suggested that 216 upregulated genes in MZ-2 were annotated by 70 GO assignments, 242 upregulated genes were associated with 188 signal pathways, while 321 upregulated genes in MZ-3 were annotated by 56 GO assignments, 322 upregulated genes were associated with 168 signal pathways. The molecular functions of upregulated genes in MZ-2 were mainly enriched for protein degradation and amino acid metabolism. The molecular functions of upregulated genes in MZ-3 were mainly enriched for transcriptional activity, cell proliferation and cell differentiation.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000408039700002
C6  - pmid:28814335
DO  - DOI:10.1186/s13071-017-2325-z
UR  - https://juser.fz-juelich.de/record/837921
ER  -