% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Hu:837937,
      author       = {Hu, Ruibo and Yu, Changjiang and Wang, Xiaoyu and Jia,
                      Chun-Lin and Pei, Shengqiang and He, Kang and He, Guo and
                      Kong, Yingzhen and Zhou, Gongke},
      title        = {{D}e novo {T}ranscriptome {A}nalysis of {M}iscanthus
                      {I}utarioriparius {I}ndentifies {C}andidate {G}enes in
                      {R}hizome {D}evelopment},
      journal      = {Frontiers in plant science},
      volume       = {8},
      issn         = {1664-462X},
      address      = {Lausanne},
      publisher    = {Frontiers Media},
      reportid     = {FZJ-2017-06698},
      pages        = {492},
      year         = {2017},
      abstract     = {Miscanthus lutarioriparius is a promising lignocellulosic
                      feedstock for second-generation bioethanol production.
                      However, the genomic resource for this species is relatively
                      limited thus hampers our understanding of the molecular
                      mechanisms underlying many important biological processes.
                      In this study, we performed the first de novo transcriptome
                      analysis of five tissues (leaf, stem, root, lateral bud and
                      rhizome bud) of M. lutarioriparius with an emphasis to
                      identify putative genes involved in rhizome development.
                      Approximately 66 gigabase (GB) paired-end clean reads were
                      obtained and assembled into 169,064 unigenes with an average
                      length of 759 bp. Among these unigenes, 103,899 $(61.5\%)$
                      were annotated in seven public protein databases.
                      Differential gene expression profiling analysis revealed
                      that 4,609, 3,188, 1,679, 1,218, and 1,077 genes were
                      predominantly expressed in root, leaf, stem, lateral bud,
                      and rhizome bud, respectively. Their expression patterns
                      were further classified into 12 distinct clusters. Pathway
                      enrichment analysis revealed that genes predominantly
                      expressed in rhizome bud were mainly involved in primary
                      metabolism and hormone signaling and transduction pathways.
                      Noteworthy, 19 transcription factors (TFs) and 16 hormone
                      signaling pathway-related genes were identified to be
                      predominantly expressed in rhizome bud compared with the
                      other tissues, suggesting putative roles in rhizome
                      formation and development. In addition, a predictive
                      regulatory network was constructed between four TFs and six
                      auxin and abscisic acid (ABA) -related genes. Furthermore,
                      the expression of 24 rhizome-specific genes was further
                      validated by quantitative real-time RT-PCR (qRT-PCR)
                      analysis. Taken together, this study provide a global
                      portrait of gene expression across five different tissues
                      and reveal preliminary insights into rhizome growth and
                      development. The data presented will contribute to our
                      understanding of the molecular mechanisms underlying rhizome
                      development in M. lutarioriparius and remarkably enrich the
                      genomic resources of Miscanthus.},
      cin          = {ER-C-1},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ER-C-1-20170209},
      pnm          = {143 - Controlling Configuration-Based Phenomena (POF3-143)},
      pid          = {G:(DE-HGF)POF3-143},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000398993000001},
      pubmed       = {pmid:28446913},
      doi          = {10.3389/fpls.2017.00492},
      url          = {https://juser.fz-juelich.de/record/837937},
}