TY  - JOUR
AU  - Koutsioumpas, Alexandros
AU  - Appavou, Marie-Sousai
AU  - Lairez, Didier
TI  - Time-Resolved Neutron Reflectivity during Supported Membrane Formation by Vesicle Fusion
JO  - Langmuir
VL  - 33
IS  - 40
SN  - 1520-5827
CY  - Washington, DC
PB  - ACS Publ.
M1  - FZJ-2017-06755
SP  - 10598
PY  - 2017
AB  - The formation of supported lipid bilayers (SLB) on hydrophilic substrates through the method of unilamelar vesicle fusion is used routinely in a wide range of biophysical studies. In an effort to control and better understand the fusion process on the substrate, many experimental studies employing different techniques have been devoted to the elucidation of the fusion mechanism. In the present work, we follow the kinetics of membrane formation using time-resolved (TR) neutron reflectivity, focusing on the structural changes near the solid/liquid interface. A clear indication of stacked bilayer structure is observed during the intermediate phase of SLB formation. Adsorbed lipid mass decrease is also measured in the final stage of the process. We have found that it is essential for the analysis of the experimental results to treat the shape of adsorbed lipid vesicles on an attractive substrate theoretically. The overall findings are discussed in relation to proposed fusion mechanisms from the literature, and we argue that our observations favor a model involving enhanced adhesion of incoming vesicles on the edges of already-formed bilayer patches.
LB  - PUB:(DE-HGF)16
C6  - pmid:28872877
UR  - <Go to ISI:>//WOS:000412966100021
DO  - DOI:10.1021/acs.langmuir.7b02459
UR  - https://juser.fz-juelich.de/record/838007
ER  -