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@ARTICLE{Koutsioumpas:838007,
author = {Koutsioumpas, Alexandros and Appavou, Marie-Sousai and
Lairez, Didier},
title = {{T}ime-{R}esolved {N}eutron {R}eflectivity during
{S}upported {M}embrane {F}ormation by {V}esicle {F}usion},
journal = {Langmuir},
volume = {33},
number = {40},
issn = {1520-5827},
address = {Washington, DC},
publisher = {ACS Publ.},
reportid = {FZJ-2017-06755},
pages = {10598},
year = {2017},
abstract = {The formation of supported lipid bilayers (SLB) on
hydrophilic substrates through the method of unilamelar
vesicle fusion is used routinely in a wide range of
biophysical studies. In an effort to control and better
understand the fusion process on the substrate, many
experimental studies employing different techniques have
been devoted to the elucidation of the fusion mechanism. In
the present work, we follow the kinetics of membrane
formation using time-resolved (TR) neutron reflectivity,
focusing on the structural changes near the solid/liquid
interface. A clear indication of stacked bilayer structure
is observed during the intermediate phase of SLB formation.
Adsorbed lipid mass decrease is also measured in the final
stage of the process. We have found that it is essential for
the analysis of the experimental results to treat the shape
of adsorbed lipid vesicles on an attractive substrate
theoretically. The overall findings are discussed in
relation to proposed fusion mechanisms from the literature,
and we argue that our observations favor a model involving
enhanced adhesion of incoming vesicles on the edges of
already-formed bilayer patches.},
cin = {JCNS (München) ; Jülich Centre for Neutron Science JCNS
(München) ; JCNS-FRM-II / Neutronenstreuung ; JCNS-1},
ddc = {670},
cid = {I:(DE-Juel1)JCNS-FRM-II-20110218 /
I:(DE-Juel1)JCNS-1-20110106},
pnm = {6215 - Soft Matter, Health and Life Sciences (POF3-621) /
6G15 - FRM II / MLZ (POF3-6G15) / 6G4 - Jülich Centre for
Neutron Research (JCNS) (POF3-623)},
pid = {G:(DE-HGF)POF3-6215 / G:(DE-HGF)POF3-6G15 /
G:(DE-HGF)POF3-6G4},
experiment = {EXP:(DE-MLZ)MARIA-20140101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:28872877},
UT = {WOS:000412966100021},
doi = {10.1021/acs.langmuir.7b02459},
url = {https://juser.fz-juelich.de/record/838007},
}