000838453 001__ 838453
000838453 005__ 20210129231545.0
000838453 037__ $$aFZJ-2017-07056
000838453 1001_ $$0P:(DE-HGF)0$$aSchlesinger, R.$$b0
000838453 245__ $$aExpression and purification of arrestin in yeast Saccharomyces cerevisiae
000838453 260__ $$aSan Diego, US$$bAcademic Press$$c2017
000838453 29510 $$aMethods in Cell Biology - G Protein-Coupled Receptors
000838453 300__ $$a159-172
000838453 3367_ $$2ORCID$$aBOOK_CHAPTER
000838453 3367_ $$07$$2EndNote$$aBook Section
000838453 3367_ $$2DRIVER$$abookPart
000838453 3367_ $$2BibTeX$$aINBOOK
000838453 3367_ $$2DataCite$$aOutput Types/Book chapter
000838453 3367_ $$0PUB:(DE-HGF)7$$2PUB:(DE-HGF)$$aContribution to a book$$bcontb$$mcontb$$s1517573697_13740
000838453 520__ $$aProtein purity and yield are two critical parameters for successful protein characterization using structural techniques such as X-ray crystallography, NMR, and several other biophysical methods. The yeast Saccharomyces cerevisiae is one of the popular eukaryotic model systems for overexpression and subsequent purification of recombinant proteins. Here, we describe a protocol for cloning, overexpression, purification, and crystallization of arrestin-1 and its splice variant p44 from yeast. The purification protocol involves a single-affinity chromatography step on a Strep-Tactin column. Highly purified arrestins can be concentrated up to 15 mg/mL using ultrafiltration and can be stored in the frozen state for several months without any loss of functionality.
000838453 536__ $$0G:(DE-HGF)POF3-551$$a551 - Functional Macromolecules and Complexes (POF3-551)$$cPOF3-551$$fPOF III$$x0
000838453 7001_ $$0P:(DE-Juel1)139572$$aCousin, Anneliese$$b1
000838453 7001_ $$0P:(DE-Juel1)131965$$aGranzin, Joachim$$b2
000838453 7001_ $$0P:(DE-Juel1)131950$$aBatra-Safferling, Renu$$b3$$eCorresponding author
000838453 909CO $$ooai:juser.fz-juelich.de:838453$$pVDB
000838453 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)139572$$aForschungszentrum Jülich$$b1$$kFZJ
000838453 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)131965$$aForschungszentrum Jülich$$b2$$kFZJ
000838453 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)131950$$aForschungszentrum Jülich$$b3$$kFZJ
000838453 9131_ $$0G:(DE-HGF)POF3-551$$1G:(DE-HGF)POF3-550$$2G:(DE-HGF)POF3-500$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bKey Technologies$$lBioSoft – Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences$$vFunctional Macromolecules and Complexes$$x0
000838453 9141_ $$y2017
000838453 920__ $$lyes
000838453 9201_ $$0I:(DE-Juel1)ICS-6-20110106$$kICS-6$$lStrukturbiochemie $$x0
000838453 980__ $$acontb
000838453 980__ $$aVDB
000838453 980__ $$aI:(DE-Juel1)ICS-6-20110106
000838453 980__ $$aUNRESTRICTED
000838453 981__ $$aI:(DE-Juel1)IBI-7-20200312