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| Hauptseite > Publikationsdatenbank > Expression and purification of arrestin in yeast Saccharomyces cerevisiae > print |
| Hauptseite > Publikationsdatenbank > Expression and purification of arrestin in yeast Saccharomyces cerevisiae > print |
| 001 | 838453 | ||
| 005 | 20210129231545.0 | ||
| 037 | _ | _ | |a FZJ-2017-07056 |
| 100 | 1 | _ | |a Schlesinger, R. |0 P:(DE-HGF)0 |b 0 |
| 245 | _ | _ | |a Expression and purification of arrestin in yeast Saccharomyces cerevisiae |
| 260 | _ | _ | |a San Diego, US |c 2017 |b Academic Press |
| 295 | 1 | 0 | |a Methods in Cell Biology - G Protein-Coupled Receptors |
| 300 | _ | _ | |a 159-172 |
| 336 | 7 | _ | |a BOOK_CHAPTER |2 ORCID |
| 336 | 7 | _ | |a Book Section |0 7 |2 EndNote |
| 336 | 7 | _ | |a bookPart |2 DRIVER |
| 336 | 7 | _ | |a INBOOK |2 BibTeX |
| 336 | 7 | _ | |a Output Types/Book chapter |2 DataCite |
| 336 | 7 | _ | |a Contribution to a book |b contb |m contb |0 PUB:(DE-HGF)7 |s 1517573697_13740 |2 PUB:(DE-HGF) |
| 520 | _ | _ | |a Protein purity and yield are two critical parameters for successful protein characterization using structural techniques such as X-ray crystallography, NMR, and several other biophysical methods. The yeast Saccharomyces cerevisiae is one of the popular eukaryotic model systems for overexpression and subsequent purification of recombinant proteins. Here, we describe a protocol for cloning, overexpression, purification, and crystallization of arrestin-1 and its splice variant p44 from yeast. The purification protocol involves a single-affinity chromatography step on a Strep-Tactin column. Highly purified arrestins can be concentrated up to 15 mg/mL using ultrafiltration and can be stored in the frozen state for several months without any loss of functionality. |
| 536 | _ | _ | |a 551 - Functional Macromolecules and Complexes (POF3-551) |0 G:(DE-HGF)POF3-551 |c POF3-551 |f POF III |x 0 |
| 700 | 1 | _ | |a Cousin, Anneliese |0 P:(DE-Juel1)139572 |b 1 |
| 700 | 1 | _ | |a Granzin, Joachim |0 P:(DE-Juel1)131965 |b 2 |
| 700 | 1 | _ | |a Batra-Safferling, Renu |0 P:(DE-Juel1)131950 |b 3 |e Corresponding author |
| 909 | C | O | |o oai:juser.fz-juelich.de:838453 |p VDB |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 1 |6 P:(DE-Juel1)139572 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 2 |6 P:(DE-Juel1)131965 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 3 |6 P:(DE-Juel1)131950 |
| 913 | 1 | _ | |a DE-HGF |b Key Technologies |l BioSoft – Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences |1 G:(DE-HGF)POF3-550 |0 G:(DE-HGF)POF3-551 |2 G:(DE-HGF)POF3-500 |v Functional Macromolecules and Complexes |x 0 |4 G:(DE-HGF)POF |3 G:(DE-HGF)POF3 |
| 914 | 1 | _ | |y 2017 |
| 920 | _ | _ | |l yes |
| 920 | 1 | _ | |0 I:(DE-Juel1)ICS-6-20110106 |k ICS-6 |l Strukturbiochemie |x 0 |
| 980 | _ | _ | |a contb |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a I:(DE-Juel1)ICS-6-20110106 |
| 980 | _ | _ | |a UNRESTRICTED |
| 981 | _ | _ | |a I:(DE-Juel1)IBI-7-20200312 |
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