001     838453
005     20210129231545.0
037 _ _ |a FZJ-2017-07056
100 1 _ |a Schlesinger, R.
|0 P:(DE-HGF)0
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245 _ _ |a Expression and purification of arrestin in yeast Saccharomyces cerevisiae
260 _ _ |a San Diego, US
|c 2017
|b Academic Press
295 1 0 |a Methods in Cell Biology - G Protein-Coupled Receptors
300 _ _ |a 159-172
336 7 _ |a BOOK_CHAPTER
|2 ORCID
336 7 _ |a Book Section
|0 7
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336 7 _ |a bookPart
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336 7 _ |a INBOOK
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336 7 _ |a Output Types/Book chapter
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336 7 _ |a Contribution to a book
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|s 1517573697_13740
|2 PUB:(DE-HGF)
520 _ _ |a Protein purity and yield are two critical parameters for successful protein characterization using structural techniques such as X-ray crystallography, NMR, and several other biophysical methods. The yeast Saccharomyces cerevisiae is one of the popular eukaryotic model systems for overexpression and subsequent purification of recombinant proteins. Here, we describe a protocol for cloning, overexpression, purification, and crystallization of arrestin-1 and its splice variant p44 from yeast. The purification protocol involves a single-affinity chromatography step on a Strep-Tactin column. Highly purified arrestins can be concentrated up to 15 mg/mL using ultrafiltration and can be stored in the frozen state for several months without any loss of functionality.
536 _ _ |a 551 - Functional Macromolecules and Complexes (POF3-551)
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|c POF3-551
|f POF III
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700 1 _ |a Cousin, Anneliese
|0 P:(DE-Juel1)139572
|b 1
700 1 _ |a Granzin, Joachim
|0 P:(DE-Juel1)131965
|b 2
700 1 _ |a Batra-Safferling, Renu
|0 P:(DE-Juel1)131950
|b 3
|e Corresponding author
909 C O |o oai:juser.fz-juelich.de:838453
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910 1 _ |a Forschungszentrum Jülich
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910 1 _ |a Forschungszentrum Jülich
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910 1 _ |a Forschungszentrum Jülich
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913 1 _ |a DE-HGF
|b Key Technologies
|l BioSoft – Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences
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|v Functional Macromolecules and Complexes
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914 1 _ |y 2017
920 _ _ |l yes
920 1 _ |0 I:(DE-Juel1)ICS-6-20110106
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980 _ _ |a contb
980 _ _ |a VDB
980 _ _ |a I:(DE-Juel1)ICS-6-20110106
980 _ _ |a UNRESTRICTED
981 _ _ |a I:(DE-Juel1)IBI-7-20200312


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