TY  - CONF
AU  - Kriehuber, Ralf
TI  - A comparative study on the cyto- and genotoxicityof I-123- and I-125-UdR in-vitro
M1  - FZJ-2017-07130
PY  - 2011
AB  - The Auger effect is not yet fully understood in respect to the deposited energy as well as to the dose rate. Therefore, we studied the Auger electron emitter (AEE) I-123 and I-125 which are characterized by a different half-life (13.2 h vs. 59.4 d) and by different average numbers of Auger electrons emitted per decay (ratio I-123/I-125 ~ 1:2). The biological response in mammalian cells labelled with various activity concentrations of 5-(123)iodine-2'-deoxyuridine (I-123-UdR) and (5-(125)iodine-2'-deoxyuridine (I-125-UdR) was thoroughly investigated to further elucidate the biological effectiveness of these particular electron emitters. SCL-II, Kidney-T1 and Jurkat cells were synchronized in G1-phase, subsequently labelled with I-123- respectively I-125-UdR and the cellular uptake and DNA incorporation of I-UdR was determined. Chromatin damage was quantified by the alkaline Comet assay, apoptosis induction assessed by the Annexin V/PI assay employing flow cytometry and micronucleus formation was quantified using the Cytochalasin-B micronucleus assay at various times post-labelling. Cs-137 γ-rays served as reference radiation.I-125-UdR induced overall a slightly stronger response in human cell lines than I-123-UdR regarding micronucleus formation and chromatin damage per decay. Both AEE caused a pronounced long-lasting G2/M phase arrest. On average one decay (I-125-UdR) every 90 seconds per DNA/cell is sufficient to induce a permanent cell cycle arrest.Albeit of a lower dose rate, I-125-UdR is slightly more genotoxic in comparison with I-123-UdR.Funded by Bundesministerium für Umwelt, Natur und Reaktorsicherheit (BMU), Bundesamt für Strahlenschutz; Project No.: 3608S03002
T2  - 7th International Symposium on Physical, Molecular, Cellular and Medical Aspects of Auger Processes
CY  - 24 Aug 2011 - 26 Aug 2011, Jülich (Germany)
Y2  - 24 Aug 2011 - 26 Aug 2011
M2  - Jülich, Germany
LB  - PUB:(DE-HGF)6
UR  - https://juser.fz-juelich.de/record/838548
ER  -