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@INPROCEEDINGS{Kriehuber:838548,
      author       = {Kriehuber, Ralf},
      title        = {{A} comparative study on the cyto- and genotoxicityof
                      {I}-123- and {I}-125-{U}d{R} in-vitro},
      reportid     = {FZJ-2017-07130},
      year         = {2011},
      abstract     = {The Auger effect is not yet fully understood in respect to
                      the deposited energy as well as to the dose rate. Therefore,
                      we studied the Auger electron emitter (AEE) I-123 and I-125
                      which are characterized by a different half-life (13.2 h vs.
                      59.4 d) and by different average numbers of Auger electrons
                      emitted per decay (ratio I-123/I-125 ~ 1:2). The biological
                      response in mammalian cells labelled with various activity
                      concentrations of 5-(123)iodine-2'-deoxyuridine (I-123-UdR)
                      and (5-(125)iodine-2'-deoxyuridine (I-125-UdR) was
                      thoroughly investigated to further elucidate the biological
                      effectiveness of these particular electron emitters. SCL-II,
                      Kidney-T1 and Jurkat cells were synchronized in G1-phase,
                      subsequently labelled with I-123- respectively I-125-UdR and
                      the cellular uptake and DNA incorporation of I-UdR was
                      determined. Chromatin damage was quantified by the alkaline
                      Comet assay, apoptosis induction assessed by the Annexin
                      V/PI assay employing flow cytometry and micronucleus
                      formation was quantified using the Cytochalasin-B
                      micronucleus assay at various times post-labelling. Cs-137
                      γ-rays served as reference radiation.I-125-UdR induced
                      overall a slightly stronger response in human cell lines
                      than I-123-UdR regarding micronucleus formation and
                      chromatin damage per decay. Both AEE caused a pronounced
                      long-lasting G2/M phase arrest. On average one decay
                      (I-125-UdR) every 90 seconds per DNA/cell is sufficient to
                      induce a permanent cell cycle arrest.Albeit of a lower dose
                      rate, I-125-UdR is slightly more genotoxic in comparison
                      with I-123-UdR.Funded by Bundesministerium für Umwelt,
                      Natur und Reaktorsicherheit (BMU), Bundesamt für
                      Strahlenschutz; Project No.: 3608S03002},
      month         = {Aug},
      date          = {2011-08-24},
      organization  = {7th International Symposium on
                       Physical, Molecular, Cellular and
                       Medical Aspects of Auger Processes,
                       Jülich (Germany), 24 Aug 2011 - 26 Aug
                       2011},
      subtyp        = {After Call},
      cin          = {S-US},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)6},
      url          = {https://juser.fz-juelich.de/record/838548},
}