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@INPROCEEDINGS{Kriehuber:838581,
      author       = {Kriehuber, Ralf},
      title        = {{C}yto- and genotoxicity of 123{I}- and 125{I}-{U}d{R} in
                      vitro: {A}poptosis induction, micronucleus formation and
                      chromatin damage in three human cell lines},
      reportid     = {FZJ-2017-07163},
      year         = {2010},
      abstract     = {The Auger electron emitters (AEE) 123I and 125I are
                      characterized by different half-lifes (13.2 h vs. 59.4 d)
                      and by different average numbers of Auger electrons emitted
                      per decay (8 vs. 15). The biological response in
                      synchronized mammalian cells labelled with various activity
                      concentrations of 123I- and 125I-UdR were investigated and
                      compared in respect to accumulated decays and dose rate to
                      further elucidate the biological effectiveness of Auger
                      electrons. SCL-II, Kidney-T1 and Jurkat cells were
                      synchronized in G1-phase, subsequently labelled with 123I-
                      respectively 125I-UdR and the cellular up-take and
                      DNA-incorporation of I-UdR were determined. Chromatin damage
                      was quantified by the alkaline Comet-assay, apoptosis
                      induction was assessed by the Annexin V/PI assay employing
                      flow cytometry and micronucleus formation was quantified
                      using the Cytochalasin-B–micronucleus assay at various
                      times post-labelling. 137Cs gamma rays served as reference
                      radiation.125I-UdR induced overall a slightly stronger
                      response in human cell lines than 123I-UdR regarding
                      micronucleus formation and chromatin damage. Apoptosis
                      induction was much more profound in 125I-UdR-labelled cells
                      immediately after labelling when compared to 123I-UdR. Both
                      AEE induced a pronounced long-lasting G2/M phase
                      arrest.Albeit of a lower dose rate, 125I-UdR is 1.2 to 1.5
                      times more genotoxic in comparison with 123I-UdR. On average
                      one decay (125I-UdR) every 120 seconds per DNA/cell is
                      sufficient to induce a permanent cell cycle arrest.},
      month         = {Sep},
      date          = {2010-09-25},
      organization  = {56th Annual Meeting of the Radiation
                       Research Society, Maui Hawaii (USA), 25
                       Sep 2010 - 29 Sep 2010},
      subtyp        = {Invited},
      cin          = {S-US},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)6},
      url          = {https://juser.fz-juelich.de/record/838581},
}