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| 001 | 838939 | ||
| 005 | 20210129231700.0 | ||
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| 100 | 1 | _ | |a Peters, J. |0 0000-0001-5151-7710 |b 0 |e Corresponding author |
| 245 | _ | _ | |a Thermodynamics of lipid multi-lamellar vesicles in presence of sterols at high hydrostatic pressure |
| 260 | _ | _ | |a London |c 2017 |b Nature Publishing Group |
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| 520 | _ | _ | |a We compared the effect of cholesterol at different concentration on the phase behaviour of DMPC(1,2-dimyristoyl-sn-glycero-3-phosphocholine) multilamellar vesicles. We used pressure perturbationdifferential scanning calorimetry (PPC) that studies a system on the whole by giving access to relevantthermodynamic quantities, and elastic incoherent neutron scattering (EINS) that probes local motionsof a system at the atomic level by allowing extraction of dynamical parameters. PPC revealed thatthe volume expansion coefficient of DMPC and DMPC/Cholesterol samples with 13 and 25 mol%cholesterol is a linear function of the heat capacity measured by differential scanning calorimetry.Neutron backscattering spectroscopy showed that the mean square displacements of H atoms doexhibit an increase with temperature and a decrease under increasing pressure. Cholesterol added atconcentrations of 25 and 50 mol% led to suppression of the main phase transition. Taking advantage ofthese results, the present study aims (i) to show that calorimetry and EINS using the Bicout and Zaccaimodel equally permit to get access to thermodynamic quantities characterizing pure DMPC and DMPC/cholesterol mixtures, thus directly confirming the theoretical method, and (ii) to validate our approachas function of temperature and of pressure, as both are equally important and complementarythermodynamic variables. |
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| 700 | 1 | _ | |a Marion, J. |0 P:(DE-HGF)0 |b 1 |
| 700 | 1 | _ | |a Becher, F. J. |0 P:(DE-HGF)0 |b 2 |
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| 700 | 1 | _ | |a Gutberlet, T. |0 P:(DE-Juel1)168124 |b 4 |u fzj |
| 700 | 1 | _ | |a Bicout, D. J. |0 0000-0003-0750-997X |b 5 |
| 700 | 1 | _ | |a Heimburg, T. |0 0000-0003-4599-4582 |b 6 |
| 773 | _ | _ | |a 10.1038/s41598-017-15582-4 |g Vol. 7, no. 1, p. 15339 |0 PERI:(DE-600)2615211-3 |n 1 |p 15339 |t Scientific reports |v 7 |y 2017 |x 2045-2322 |
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