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@INPROCEEDINGS{Heine:839898,
      author       = {Heine and Jaeger, Alexandra and Pomplun, Ekkehard and
                      Kriehuber, Ralf},
      title        = {{LACK} {OF} {BYSTANDER} {EFFECTS} {IN} {TWO} {MAMMALIAN}
                      {CELL} {LINES} {AFTER} {EXPOSURE} {TO} {THE} {AUGER}
                      {ELECTRON} {EMITTER} {I}-123- {AND} {I}-125},
      reportid     = {FZJ-2017-07476},
      year         = {2011},
      abstract     = {Introduction: Non-targeted effects caused by signalling of
                      directly irradiated cells to neighboring non-irradiated
                      cells, termed “bystander effect”, is still
                      controversially discussed, especially in radiotherapy. Xue
                      et al. (2002) demonstrated that the subcutaneous injection
                      into nude mice of human adenocarcinoma LS174T cells lethally
                      irradiated by Auger electrons from the decay of
                      DNA-incorporated I-125 inhibits growth of co-injected LS174T
                      cells (inhibitory bystander effect). Kishikawa et al. (2006)
                      from the same group demonstrated in the same experimental
                      set-up that I-123, an Auger electron emitter (AEE) whose
                      emission spectrum is similar to that of I-125, stimulates
                      the proliferation of neighboring unlabeled tumor cells
                      (stimulatory bystander effect) and attributed the effect to
                      soluble factors. To elucidate the nature of the opposed
                      bystander effect for I-123 and I-125, we studied the cell
                      culture medium-mediated bystander effect in respect to
                      micronucleus formation and apoptosis in two mammalian cell
                      lines.Methods: Medium from AEE-treated human SCL-II and
                      Kidney-T1 cells (I-123-UdR: 16, 48, 80 kBq/ml; I-125-UdR: 8,
                      24, 40 kBq/ml) was harvested and transferred to the
                      respective non-irradiated bystander cell cultures.
                      Micronucleus formation and apoptosis induction was assessed
                      by fluorescence microscopy (Hoechst-33342 DNA staining)
                      respectively flow cytometry (Annexin V/PI assay) for up to
                      48 h.Results: No significant bystander effect in respect to
                      micronucleus formation and apoptosis induction in neither
                      cell-line was observed.Conclusions: Neither I-123 nor I-125
                      have been found to induce cell culture medium-mediated
                      bystander effects in the two investigated cell lines.
                      However, in consideration of publications with contrary
                      findings, the specific cell line respectively culture or
                      experimental conditions may be of importance for inducing
                      bystander effects. Other than stated the reported findings
                      by Xue et al. (2002) and Kishikawa et al. (2006) might also
                      be due to cell-cell contact mediated bystander effects.
                      Funded by Bundesamt für Strahlenschutz; Bundesministerium
                      für Umwelt, Natur und Reaktorsicherheit (BMU); Project No.:
                      3608S03002Referenzen:Xue et al. Proc. Natl. Acad. Sci. USA
                      99, 13765-13770, 2002Kishikawa et al. Radiat.
                      Res.;165(6):688-94, 2006},
      month         = {Sep},
      date          = {2011-09-13},
      organization  = {14. Jahrestagung der Geselllschaft
                       für Biologische Strahlenforschung,
                       Rheinbach (Germany), 13 Sep 2011 - 16
                       Sep 2011},
      subtyp        = {After Call},
      cin          = {S-US},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)24},
      url          = {https://juser.fz-juelich.de/record/839898},
}