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@INPROCEEDINGS{Oskamp:839902,
      author       = {Oskamp, Dominik and Jaeger, Alexandra and Pomplun, Ekkehard
                      and Kriehuber, Ralf},
      title        = {{CHARACTERIZATION} {OF} {CELL} {CYCLE} {PERTURBANCES} {IN}
                      {SCL}-{II} {CELLS} {AFTER} {EXPOSURE} {TO} {THE} {AUGER}
                      {ELECTRON} {EMITTER} {I}-125},
      reportid     = {FZJ-2017-07480},
      year         = {2011},
      abstract     = {Introduction: Theoretical calculations and experimental
                      findings suggest that DNA-incorporated Auger electron
                      emitter (AEE) cause primarily complex DNA lesions. Recent
                      cell cycle analysis revealed, that
                      5-(125)iodine-2'-deoxyuridine (I-125-UdR)-exposed SCL-II
                      cells display a pronounced and rather long-lasting G2/M cell
                      cycle arrest. We studied therefore in more detail whether
                      the observed G2/M arrest is of permanent or temporary nature
                      and thereby examined the average number of decay per cell
                      necessary to induce a long-lasting cell cycle arrest in
                      SCL-II cells.Methods: SCL-II cells were synchronized in
                      G1-phase and incubated with 0.1, 1, 4 and 8 kBq/ml of
                      I-125-UdR during S-phase so that approximately 90 $\%$ of
                      the cells were subsequently labeled. Cell cycle analysis was
                      performed by flow cytometry (Click-iT EdU cell proliferation
                      assay and Sytox Green nucleic acid stain) up to 4 d
                      post-labeling with a constant sampling every 8 h
                      post-labeling.Results: DNA-incorporated I-125-UdR
                      decelerated SCL-II cell cycle progression. Cell cycle
                      perturbances were observed and a pronounced and prolonged
                      G2/M-phase arrest was detected at activity concentration of
                      4 kBq/ml I-125-UdR. Up to 70 $\%$ of the cells were arrested
                      in G2/M-phase up to 38 h post-labeling with 8 kBq/ml
                      I-125-UdR. Moreover, a distinct population of G2/M-arrested
                      cells was detectable up to 94 h post-labeling. As shown by
                      parallel EdU staining these cells were permanently caught in
                      the first G2/M cell cycle phase post-labeling. On average
                      one decay every 90 s per DNA/cell was calculated to induce a
                      permanent G2/M arrest in SCL-II cells.Conclusions:
                      Incorporated I-125-UdR induces major cell cycle perturbances
                      in SCL-II cells. The observed permanent G2/M arrest suggests
                      a threshold in terms of decays per time per cell and hence
                      persisting DNA damage beyond no G2/M release seems to occur.
                      This implies different levels of DNA damage for the
                      induction and the release of the G2/M arrest in SCL-II
                      cells. Funded by Bundesministerium für Umwelt, Natur und
                      Reaktorsicherheit (BMU), Bundesamt für Strahlenschutz;
                      Project No.: 3608S03002},
      month         = {Sep},
      date          = {2011-09-13},
      organization  = {14. Jahrestagung der Gesellschaft für
                       Biologische Strahlenforschung,
                       Rheinbach (Germany), 13 Sep 2011 - 16
                       Sep 2011},
      subtyp        = {After Call},
      cin          = {S-US},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)24},
      url          = {https://juser.fz-juelich.de/record/839902},
}