Gast ::
| Hauptseite > Publikationsdatenbank > CHARACTERIZATION OF CELL CYCLE PERTURBANCES IN SCL-II CELLS AFTER EXPOSURE TO THE AUGER ELECTRON EMITTER I-125 > print |
| Hauptseite > Publikationsdatenbank > CHARACTERIZATION OF CELL CYCLE PERTURBANCES IN SCL-II CELLS AFTER EXPOSURE TO THE AUGER ELECTRON EMITTER I-125 > print |
| 001 | 839902 | ||
| 005 | 20210129231706.0 | ||
| 024 | 7 | _ | |a 2128/15863 |2 Handle |
| 037 | _ | _ | |a FZJ-2017-07480 |
| 041 | _ | _ | |a English |
| 100 | 1 | _ | |a Oskamp, Dominik |0 P:(DE-Juel1)133339 |b 0 |e Corresponding author |u fzj |
| 111 | 2 | _ | |a 14. Jahrestagung der Gesellschaft für Biologische Strahlenforschung |g GBS |c Rheinbach |d 2011-09-13 - 2011-09-16 |w Germany |
| 245 | _ | _ | |a CHARACTERIZATION OF CELL CYCLE PERTURBANCES IN SCL-II CELLS AFTER EXPOSURE TO THE AUGER ELECTRON EMITTER I-125 |
| 260 | _ | _ | |c 2011 |
| 336 | 7 | _ | |a Conference Paper |0 33 |2 EndNote |
| 336 | 7 | _ | |a INPROCEEDINGS |2 BibTeX |
| 336 | 7 | _ | |a conferenceObject |2 DRIVER |
| 336 | 7 | _ | |a CONFERENCE_POSTER |2 ORCID |
| 336 | 7 | _ | |a Output Types/Conference Poster |2 DataCite |
| 336 | 7 | _ | |a Poster |b poster |m poster |0 PUB:(DE-HGF)24 |s 1510581101_28470 |2 PUB:(DE-HGF) |x After Call |
| 520 | _ | _ | |a Introduction: Theoretical calculations and experimental findings suggest that DNA-incorporated Auger electron emitter (AEE) cause primarily complex DNA lesions. Recent cell cycle analysis revealed, that 5-(125)iodine-2'-deoxyuridine (I-125-UdR)-exposed SCL-II cells display a pronounced and rather long-lasting G2/M cell cycle arrest. We studied therefore in more detail whether the observed G2/M arrest is of permanent or temporary nature and thereby examined the average number of decay per cell necessary to induce a long-lasting cell cycle arrest in SCL-II cells.Methods: SCL-II cells were synchronized in G1-phase and incubated with 0.1, 1, 4 and 8 kBq/ml of I-125-UdR during S-phase so that approximately 90 % of the cells were subsequently labeled. Cell cycle analysis was performed by flow cytometry (Click-iT EdU cell proliferation assay and Sytox Green nucleic acid stain) up to 4 d post-labeling with a constant sampling every 8 h post-labeling.Results: DNA-incorporated I-125-UdR decelerated SCL-II cell cycle progression. Cell cycle perturbances were observed and a pronounced and prolonged G2/M-phase arrest was detected at activity concentration of 4 kBq/ml I-125-UdR. Up to 70 % of the cells were arrested in G2/M-phase up to 38 h post-labeling with 8 kBq/ml I-125-UdR. Moreover, a distinct population of G2/M-arrested cells was detectable up to 94 h post-labeling. As shown by parallel EdU staining these cells were permanently caught in the first G2/M cell cycle phase post-labeling. On average one decay every 90 s per DNA/cell was calculated to induce a permanent G2/M arrest in SCL-II cells.Conclusions: Incorporated I-125-UdR induces major cell cycle perturbances in SCL-II cells. The observed permanent G2/M arrest suggests a threshold in terms of decays per time per cell and hence persisting DNA damage beyond no G2/M release seems to occur. This implies different levels of DNA damage for the induction and the release of the G2/M arrest in SCL-II cells. Funded by Bundesministerium für Umwelt, Natur und Reaktorsicherheit (BMU), Bundesamt für Strahlenschutz; Project No.: 3608S03002 |
| 536 | _ | _ | |a 899 - ohne Topic (POF3-899) |0 G:(DE-HGF)POF3-899 |c POF3-899 |f POF III |x 0 |
| 650 | 2 | 7 | |a Biology |0 V:(DE-MLZ)SciArea-160 |2 V:(DE-HGF) |x 0 |
| 700 | 1 | _ | |a Jaeger, Alexandra |0 P:(DE-Juel1)142514 |b 1 |
| 700 | 1 | _ | |a Pomplun, Ekkehard |0 P:(DE-Juel1)133341 |b 2 |
| 700 | 1 | _ | |a Kriehuber, Ralf |0 P:(DE-Juel1)133469 |b 3 |u fzj |
| 856 | 4 | _ | |y OpenAccess |u https://juser.fz-juelich.de/record/839902/files/FZJ-2014-03957_poster.pdf |
| 856 | 4 | _ | |y OpenAccess |x icon |u https://juser.fz-juelich.de/record/839902/files/FZJ-2014-03957_poster.gif?subformat=icon |
| 856 | 4 | _ | |y OpenAccess |x icon-1440 |u https://juser.fz-juelich.de/record/839902/files/FZJ-2014-03957_poster.jpg?subformat=icon-1440 |
| 856 | 4 | _ | |y OpenAccess |x icon-180 |u https://juser.fz-juelich.de/record/839902/files/FZJ-2014-03957_poster.jpg?subformat=icon-180 |
| 856 | 4 | _ | |y OpenAccess |x icon-640 |u https://juser.fz-juelich.de/record/839902/files/FZJ-2014-03957_poster.jpg?subformat=icon-640 |
| 856 | 4 | _ | |y OpenAccess |x pdfa |u https://juser.fz-juelich.de/record/839902/files/FZJ-2014-03957_poster.pdf?subformat=pdfa |
| 909 | C | O | |o oai:juser.fz-juelich.de:839902 |p openaire |p open_access |p VDB |p driver |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 0 |6 P:(DE-Juel1)133339 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 3 |6 P:(DE-Juel1)133469 |
| 913 | 1 | _ | |a DE-HGF |b Programmungebundene Forschung |l ohne Programm |1 G:(DE-HGF)POF3-890 |0 G:(DE-HGF)POF3-899 |2 G:(DE-HGF)POF3-800 |v ohne Topic |x 0 |4 G:(DE-HGF)POF |3 G:(DE-HGF)POF3 |
| 915 | _ | _ | |a OpenAccess |0 StatID:(DE-HGF)0510 |2 StatID |
| 920 | _ | _ | |l yes |
| 920 | 1 | _ | |0 I:(DE-Juel1)S-US-20090406 |k S-US |l Sicherheit und Strahlenschutz, Umgebungsüberwachung,Strahlenbiologie |x 0 |
| 980 | _ | _ | |a poster |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a UNRESTRICTED |
| 980 | _ | _ | |a I:(DE-Juel1)S-US-20090406 |
| 980 | 1 | _ | |a FullTexts |
| Library | Collection | CLSMajor | CLSMinor | Language | Author |
|---|