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@INPROCEEDINGS{Kriehuber:839905,
      author       = {Kriehuber, Ralf and Jaeger, Alexandra and Oskamp, Dominik
                      and Pomplun, Ekkehard},
      title        = {{A} comparative study on the cyto- and genotoxicity of the
                      {A}uger electron emitter {I}-123- and {I}-125 in-vitro},
      reportid     = {FZJ-2017-07483},
      year         = {2012},
      abstract     = {The Auger effect is not yet fully understood in respect to
                      the deposited energy as well as to the dose rate. Therefore,
                      we studied the Auger electron emitter (AEE) I-123 and I-125
                      which are characterized by a different half-life (13.2 h vs.
                      59.4 d) and by different average numbers of Auger electrons
                      emitted per decay (ratio I-123/I-125 ~ 1:2). The biological
                      response in mammalian cells labelled with various activity
                      concentrations of 5-(123)iodine-2'-deoxyuridine (I-123-UdR)
                      and (5-(125)iodine-2'-deoxyuridine (I-125-UdR) was
                      thoroughly investigated to further elucidate the biological
                      effectiveness of these particular electron emitters. SCL-II
                      cells were synchronized in G1-cell cycle phase, subsequently
                      labelled with I-123- respectively I-125-UdR and the cellular
                      uptake and DNA incorporation of I-UdR was determined.
                      Chromatin damage was quantified by the alkaline Comet assay,
                      apoptosis induction assessed by the Annexin V/PI assay
                      employing flow cytometry and micronucleus formation was
                      quantified using the Cytochalasin-B micronucleus assay at
                      various times post-labelling. Cs-137 γ-rays served as
                      reference radiation.I-125-UdR caused pronounced apoptosis
                      when compared to !-123-UdR. Micronucleus induction and
                      chromatin damage was very similar for both radionuclides.
                      Both AEE caused a pronounced and long-lasting G2/M cell
                      cycle arrest. On average one decay of I-125 every 120
                      seconds in the DNA of a single cell is sufficient to induce
                      a permanent G2/M cell cycle arrest in SCL-II cells.Albeit of
                      a lower dose rate, I-125-UdR is more cytotoxic in comparison
                      with I-123-UdR.Funded by Bundesministerium für Umwelt,
                      Natur und Reaktorsicherheit (BMU), Bundesamt für
                      Strahlenschutz; Project No.: 3608S03002},
      month         = {May},
      date          = {2012-05-13},
      organization  = {13th International Congress of the
                       International Radiation Protection
                       Association, Glasgow (UK), 13 May 2012
                       - 18 May 2012},
      subtyp        = {After Call},
      cin          = {S-US},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)24},
      url          = {https://juser.fz-juelich.de/record/839905},
}