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@ARTICLE{Qi:8400,
author = {Qi, Y. and Dhiman, H.K. and Bhola, N.E. and Budyak, I. and
Kar, S. and Man, D. and Dutta, A. and Tirupula, K. and Carr,
B. and Grandis, J.R. and Bar-Joseph, Z. and
Klein-Seetharaman, J.},
title = {{S}ystematic prediction of human membrane receptor
interactions},
journal = {Proteomics},
volume = {9},
issn = {1615-9853},
address = {Weinheim},
publisher = {Wiley VCH},
reportid = {PreJuSER-8400},
pages = {5243 - 5255},
year = {2009},
note = {This work was supported in part by National Science
Foundation Grants ITR 0225656, CAREER 0448453 and CAREER
CC044917, National Institutes of Health Grants NLM108730,
R01 CA098372 and AI060422 and the Sofya Kovalvskaya Award
(to J. K. S.) from the Humboldt Foundation.},
abstract = {Membrane receptor-activated signal transduction pathways
are integral to cellular functions and disease mechanisms in
humans. Identification of the full set of proteins
interacting with membrane receptors by high-throughput
experimental means is difficult because methods to directly
identify protein interactions are largely not applicable to
membrane proteins. Unlike prior approaches that attempted to
predict the global human interactome, we used a
computational strategy that only focused on discovering the
interacting partners of human membrane receptors leading to
improved results for these proteins. We predict specific
interactions based on statistical integration of biological
data containing highly informative direct and indirect
evidences together with feedback from experts. The predicted
membrane receptor interactome provides a system-wide view,
and generates new biological hypotheses regarding
interactions between membrane receptors and other proteins.
We have experimentally validated a number of these
interactions. The results suggest that a framework of
systematically integrating computational predictions, global
analyses, biological experimentation and expert feedback is
a feasible strategy to study the human membrane receptor
interactome.},
keywords = {Computational Biology: methods / Humans / Protein
Interaction Mapping: methods / Proteome: analysis /
Proteome: metabolism / Proteomics: methods / Receptor,
Epidermal Growth Factor: analysis / Receptor, Epidermal
Growth Factor: metabolism / Receptors, Cell Surface:
analysis / Receptors, Cell Surface: metabolism / Signal
Transduction / Systems Biology: methods / Proteome (NLM
Chemicals) / Receptors, Cell Surface (NLM Chemicals) / EGFR
protein, human (NLM Chemicals) / Receptor, Epidermal Growth
Factor (NLM Chemicals) / J (WoSType)},
cin = {ISB-2},
ddc = {540},
cid = {I:(DE-Juel1)ISB-2-20090406},
pnm = {Programm Biosoft},
pid = {G:(DE-Juel1)FUEK443},
shelfmark = {Biochemical Research Methods / Biochemistry $\&$ Molecular
Biology},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:19798668},
pmc = {pmc:PMC3076061},
UT = {WOS:000273393900006},
doi = {10.1002/pmic.200900259},
url = {https://juser.fz-juelich.de/record/8400},
}