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@ARTICLE{Wang:840140,
author = {Wang, Liming and Amelung, Wulf and Willbold, Sabine},
title = {{D}iffusion-{O}rdered {N}uclear {M}agnetic {R}esonance
{S}pectroscopy ({DOSY}-{NMR}): {A} {N}ovel {T}ool for
{I}dentification of {P}hosphorus {C}ompounds in {S}oil
{E}xtracts},
journal = {Environmental science $\&$ technology},
volume = {51},
number = {22},
issn = {1520-5851},
address = {Columbus, Ohio},
publisher = {American Chemical Society},
reportid = {FZJ-2017-07699},
pages = {13256 - 13264},
year = {2017},
abstract = {Liquid-state, one-dimension 31P nuclear magnetic resonance
spectroscopy (NMR) has greatly advanced our understanding of
the composition of organic phosphorus in the environment.
However, the correct assignment of signals is complicated by
overlapping and shifting signals in different types of
soils. We applied therefore for the first time
diffusion-ordered spectroscopy (DOSY) to soil extracts,
allowing us to separate phosphorus components in the second
domain based on their translational diffusion coefficients.
After successful application to a mixture of 14 model
compounds, diffusion rates correlated closely with the
molecular weight of the individual compound in aqueous
solution (R2 = 0.97). The method was then applied to
NaOH/EDTA extracts of a grassland soil, of which
paramagnetic contaminations were removed with sodium sulfide
following high-velocity centrifugation (21 500g, 45 min)
at 4 °C. Diffusion rates in soil extracts were again
closely related to molecular weight (R2 = 0.98), varying
from 163.9 to 923.8 Da. However, our DOSY application failed
for a forest soil with low organic phosphorus content.
Overall, DOSY did help to clearly identify specific NMR
signals like myo- and scyllo-inositol hexakisphosphate. It
thus provides a more confident signal assignment than 1D 31P
NMR, although currently the ubiquitous use of this novel
methodology is still limited to soils with high organic
phosphorus content.},
cin = {ZEA-3 / IBG-3},
ddc = {050},
cid = {I:(DE-Juel1)ZEA-3-20090406 / I:(DE-Juel1)IBG-3-20101118},
pnm = {255 - Terrestrial Systems: From Observation to Prediction
(POF3-255) / HITEC - Helmholtz Interdisciplinary Doctoral
Training in Energy and Climate Research (HITEC)
(HITEC-20170406)},
pid = {G:(DE-HGF)POF3-255 / G:(DE-Juel1)HITEC-20170406},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:29065692},
UT = {WOS:000416496700022},
doi = {10.1021/acs.est.7b03322},
url = {https://juser.fz-juelich.de/record/840140},
}