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@ARTICLE{Eichner:840625,
author = {Eichner, Adina and Stahlberg, Sören and Sonnenberger,
Stefan and Lange, Stefan and Dobner, Bodo and Ostermann,
Andreas and Schrader, Tobias E. and Hauß, Thomas and
Schroeter, Annett and Huster, Daniel and Neubert, Reinhard
H. H.},
title = {{I}nfluence of the penetration enhancer isopropyl myristate
on stratum corneum lipid model membranes revealed by neutron
diffraction and 2 {H} {NMR} experiments},
journal = {Biochimica et biophysica acta / Biomembranes},
volume = {1859},
number = {5},
issn = {0005-2736},
address = {Amsterdam},
publisher = {Elsevier},
reportid = {FZJ-2017-08130},
pages = {745 - 755},
year = {2017},
abstract = {The stratum corneum (SC) provides the main barrier
properties in native skin. The barrier function is
attributed to the intercellular lipids, forming continuous
multilamellar membranes. In this study, SC lipid membranes
in model ratios were enriched with deuterated lipids in
order to investigate structural and dynamical properties by
neutron diffraction and 2H solid-state NMR spectroscopy.
Further, the effect of the penetration enhancer isopropyl
myristate (IPM) on the structure of a well-known SC lipid
model membrane containing synthetically derived
methyl-branched ceramide [EOS], ceramide [AP], behenic acid
and cholesterol (23/10/33/33 $wt\%)$ was investigated. IPM
supported the formation of a single short-periodicity phase
(SPP), in which we determined the molecular organization of
CER[AP] and CER[EOS]-br for the first time. Furthermore, the
thermotropic phase behavior of the lipid system was analyzed
by additional neutron diffraction studies as well as by 2H
solid-state NMR spectroscopy, covering temperatures of 32
°C (physiological skin temperature), 50 °C, and 70 °C
with a subsequent cooldown back to skin temperature. Both
techniques revealed a phase transition and a hysteresis
effect. During the cooldown, Bragg peaks corresponding to a
long-periodicity phase (LPP) appeared. Additionally, 2H NMR
revealed that the IPM molecules are isotopic mobile at all
temperatures.},
cin = {JCNS (München) ; Jülich Centre for Neutron Science JCNS
(München) ; JCNS-FRM-II / Neutronenstreuung ; JCNS-1},
ddc = {570},
cid = {I:(DE-Juel1)JCNS-FRM-II-20110218 /
I:(DE-Juel1)JCNS-1-20110106},
pnm = {6G15 - FRM II / MLZ (POF3-6G15) / 6G4 - Jülich Centre for
Neutron Research (JCNS) (POF3-623)},
pid = {G:(DE-HGF)POF3-6G15 / G:(DE-HGF)POF3-6G4},
experiment = {EXP:(DE-MLZ)BIODIFF-20140101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:28132900},
UT = {WOS:000398870800010},
doi = {10.1016/j.bbamem.2017.01.029},
url = {https://juser.fz-juelich.de/record/840625},
}