TY  - JOUR
AU  - Midtgaard, Søren Roi
AU  - Darwish, Tamim A.
AU  - Pedersen, Martin Cramer
AU  - Huda, Pie
AU  - Larsen, Andreas Haahr
AU  - Jensen, Grethe Vestergaard
AU  - Kynde, Søren Andreas Røssel
AU  - Skar-Gislinge, Nicholas
AU  - Nielsen, Agnieszka Janina Zygadlo
AU  - Olesen, Claus
AU  - Blaise, Mickael
AU  - Dorosz, Jerzy Józef
AU  - Thorsen, Thor Seneca
AU  - Venskutonytė, Raminta
AU  - Krintel, Christian
AU  - Møller, Jesper V.
AU  - Frielinghaus, Henrich
AU  - Gilbert, Elliot Paul
AU  - Martel, Anne
AU  - Kastrup, Jette Sandholm
AU  - Jensen, Poul Erik
AU  - Nissen, Poul
AU  - Arleth, Lise
TI  - Invisible detergents for structure determination of membrane proteins by small-angle neutron scattering
JO  - The FEBS journal
VL  - 285
IS  - 2
SN  - 1742-464X
CY  - Oxford [u.a.]
PB  - Wiley-Blackwell
M1  - FZJ-2017-08171
SP  - 357–371
PY  - 2018
AB  - A novel and generally applicable method for determining structures of membrane proteins in solution via small-angle neutron scattering (SANS) is presented. Common detergents for solubilizing membrane proteins were synthesized in isotope-substituted versions for utilizing the intrinsic neutron scattering length difference between hydrogen and deuterium. Individual hydrogen/deuterium levels of the detergent head and tail groups were achieved such that the formed micelles became effectively invisible in heavy water (D2O) when investigated by neutrons. This way, only the signal from the membrane protein remained in the SANS data. We demonstrate that the method is not only generally applicable on five very different membrane proteins but also reveals subtle structural details about the sarco/endoplasmatic reticulum Ca2+ ATPase (SERCA). In all, the synthesis of isotope-substituted detergents makes solution structure determination of membrane proteins bySANS and subsequent data analysis available to non-specialists.
LB  - PUB:(DE-HGF)16
C6  - pmid:29178440
UR  - <Go to ISI:>//WOS:000423416700011
DO  - DOI:10.1111/febs.14345
UR  - https://juser.fz-juelich.de/record/841071
ER  -