Home > Publications database > Invisible detergents for structure determination of membrane proteins by small-angle neutron scattering > print

001     841071
005     20210129231905.0
024 7 _ |a 10.1111/febs.14345
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024 7 _ |a 1432-1033
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024 7 _ |a 1742-464X
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024 7 _ |a 2128/17043
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100 1 _ |a Midtgaard, Søren Roi
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245 _ _ |a Invisible detergents for structure determination of membrane proteins by small-angle neutron scattering
260 _ _ |a Oxford [u.a.]
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|b Wiley-Blackwell
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520 _ _ |a A novel and generally applicable method for determining structures of membrane proteins in solution via small-angle neutron scattering (SANS) is presented. Common detergents for solubilizing membrane proteins were synthesized in isotope-substituted versions for utilizing the intrinsic neutron scattering length difference between hydrogen and deuterium. Individual hydrogen/deuterium levels of the detergent head and tail groups were achieved such that the formed micelles became effectively invisible in heavy water (D2O) when investigated by neutrons. This way, only the signal from the membrane protein remained in the SANS data. We demonstrate that the method is not only generally applicable on five very different membrane proteins but also reveals subtle structural details about the sarco/endoplasmatic reticulum Ca2+ ATPase (SERCA). In all, the synthesis of isotope-substituted detergents makes solution structure determination of membrane proteins bySANS and subsequent data analysis available to non-specialists.
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693 _ _ |a Forschungs-Neutronenquelle Heinz Maier-Leibnitz
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700 1 _ |a Darwish, Tamim A.
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700 1 _ |a Pedersen, Martin Cramer
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700 1 _ |a Huda, Pie
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700 1 _ |a Larsen, Andreas Haahr
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700 1 _ |a Jensen, Grethe Vestergaard
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700 1 _ |a Kynde, Søren Andreas Røssel
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700 1 _ |a Skar-Gislinge, Nicholas
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700 1 _ |a Nielsen, Agnieszka Janina Zygadlo
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700 1 _ |a Olesen, Claus
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700 1 _ |a Blaise, Mickael
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700 1 _ |a Dorosz, Jerzy Józef
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700 1 _ |a Thorsen, Thor Seneca
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700 1 _ |a Venskutonytė, Raminta
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700 1 _ |a Krintel, Christian
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700 1 _ |a Møller, Jesper V.
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700 1 _ |a Gilbert, Elliot Paul
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700 1 _ |a Martel, Anne
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700 1 _ |a Kastrup, Jette Sandholm
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700 1 _ |a Jensen, Poul Erik
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700 1 _ |a Nissen, Poul
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700 1 _ |a Arleth, Lise
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