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000841251 041__ $$aEnglish
000841251 1001_ $$0P:(DE-Juel1)133466$$aUnverricht, Marcus$$b0$$eCorresponding author$$ufzj
000841251 1112_ $$a3rd European International Radiation Protection Association$$cHelsinki$$d2010-06-14 - 2010-06-18$$gIRPA$$wFinland
000841251 245__ $$aQuantification of γ-H2AX foci following α-particles and γ-rays in Jurkat cells
000841251 260__ $$c2010
000841251 3367_ $$033$$2EndNote$$aConference Paper
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000841251 520__ $$aOBJECTIVES: Phosphorylation of histone H2AX occurs at sites flanking DNA double-strand breaks (DSBs) and can provide a measure of the number of DSBs within a cell. We investigated whether the mean intensity and the mean number of radiation-induced γ-H2AX foci per cell vary as a function of radiation quality and dose. Furthermore we investigated whether the mean intensity and/or the mean number of radiation-induced γ-H2AX foci is correlated with the induction of apoptosis. MATERIALS AND METHODS: Jurkat cells were irradiated with different doses of either low linear energy transfer (LET) 137Cs γ-rays or high LET 241Am α-particles. The γ-H2AX foci were detected using immunocytochemistry and quantified by measuring the mean signal intensity of γ-H2AX foci  per cell using flow cytometry and by counting the number of γ-H2AX foci with a fluorescence microscope. Apoptosis 24h after irradiation was detected via Annexin-V-FITC/ PI-assay. RESULTS: The mean number of γ-H2AX foci per cell increase with dose for both radiation qualities and are fairly identical at 1 Gy. The mean intensity of γ-H2AX foci after α-irradiation is significantly increased when compared to γ-irradiation at the same radiation dose. α-particles induce more apoptosis than γ-rays at the same dose and at a similar mean number of radiation-induced γ-H2AX foci per cell. CONCLUSION: The induction of apoptosis in jurkat cells as well as the mean signal intensity, but not the mean number, of γ-H2AX foci per cell depends on the LET.
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000841251 7001_ $$0P:(DE-HGF)0$$aGiesen, Ulrich$$b1
000841251 7001_ $$0P:(DE-Juel1)133469$$aKriehuber, Ralf$$b2$$ufzj
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