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@INPROCEEDINGS{Unverricht:841251,
      author       = {Unverricht, Marcus and Giesen, Ulrich and Kriehuber, Ralf},
      title        = {{Q}uantification of γ-{H}2{AX} foci following α-particles
                      and γ-rays in {J}urkat cells},
      reportid     = {FZJ-2017-08343},
      year         = {2010},
      abstract     = {OBJECTIVES: Phosphorylation of histone H2AX occurs at sites
                      flanking DNA double-strand breaks (DSBs) and can provide a
                      measure of the number of DSBs within a cell. We investigated
                      whether the mean intensity and the mean number of
                      radiation-induced γ-H2AX foci per cell vary as a function
                      of radiation quality and dose. Furthermore we investigated
                      whether the mean intensity and/or the mean number of
                      radiation-induced γ-H2AX foci is correlated with the
                      induction of apoptosis. MATERIALS AND METHODS: Jurkat cells
                      were irradiated with different doses of either low linear
                      energy transfer (LET) 137Cs γ-rays or high LET 241Am
                      α-particles. The γ-H2AX foci were detected using
                      immunocytochemistry and quantified by measuring the mean
                      signal intensity of γ-H2AX foci per cell using flow
                      cytometry and by counting the number of γ-H2AX foci with a
                      fluorescence microscope. Apoptosis 24h after irradiation was
                      detected via Annexin-V-FITC/ PI-assay. RESULTS: The mean
                      number of γ-H2AX foci per cell increase with dose for both
                      radiation qualities and are fairly identical at 1 Gy. The
                      mean intensity of γ-H2AX foci after α-irradiation is
                      significantly increased when compared to γ-irradiation at
                      the same radiation dose. α-particles induce more apoptosis
                      than γ-rays at the same dose and at a similar mean number
                      of radiation-induced γ-H2AX foci per cell. CONCLUSION: The
                      induction of apoptosis in jurkat cells as well as the mean
                      signal intensity, but not the mean number, of γ-H2AX foci
                      per cell depends on the LET.},
      month         = {Jun},
      date          = {2010-06-14},
      organization  = {3rd European International Radiation
                       Protection Association, Helsinki
                       (Finland), 14 Jun 2010 - 18 Jun 2010},
      subtyp        = {After Call},
      cin          = {S-US},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)6},
      url          = {https://juser.fz-juelich.de/record/841251},
}