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| Home > Publications database > Quantification of γ-H2AX foci following α-particles and γ-rays in Jurkat cells > print |
| 001 | 841251 | ||
| 005 | 20210129232004.0 | ||
| 024 | 7 | _ | |a 2128/16214 |2 Handle |
| 037 | _ | _ | |a FZJ-2017-08343 |
| 041 | _ | _ | |a English |
| 100 | 1 | _ | |a Unverricht, Marcus |0 P:(DE-Juel1)133466 |b 0 |e Corresponding author |u fzj |
| 111 | 2 | _ | |a 3rd European International Radiation Protection Association |g IRPA |c Helsinki |d 2010-06-14 - 2010-06-18 |w Finland |
| 245 | _ | _ | |a Quantification of γ-H2AX foci following α-particles and γ-rays in Jurkat cells |
| 260 | _ | _ | |c 2010 |
| 336 | 7 | _ | |a Conference Paper |0 33 |2 EndNote |
| 336 | 7 | _ | |a Other |2 DataCite |
| 336 | 7 | _ | |a INPROCEEDINGS |2 BibTeX |
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| 520 | _ | _ | |a OBJECTIVES: Phosphorylation of histone H2AX occurs at sites flanking DNA double-strand breaks (DSBs) and can provide a measure of the number of DSBs within a cell. We investigated whether the mean intensity and the mean number of radiation-induced γ-H2AX foci per cell vary as a function of radiation quality and dose. Furthermore we investigated whether the mean intensity and/or the mean number of radiation-induced γ-H2AX foci is correlated with the induction of apoptosis. MATERIALS AND METHODS: Jurkat cells were irradiated with different doses of either low linear energy transfer (LET) 137Cs γ-rays or high LET 241Am α-particles. The γ-H2AX foci were detected using immunocytochemistry and quantified by measuring the mean signal intensity of γ-H2AX foci per cell using flow cytometry and by counting the number of γ-H2AX foci with a fluorescence microscope. Apoptosis 24h after irradiation was detected via Annexin-V-FITC/ PI-assay. RESULTS: The mean number of γ-H2AX foci per cell increase with dose for both radiation qualities and are fairly identical at 1 Gy. The mean intensity of γ-H2AX foci after α-irradiation is significantly increased when compared to γ-irradiation at the same radiation dose. α-particles induce more apoptosis than γ-rays at the same dose and at a similar mean number of radiation-induced γ-H2AX foci per cell. CONCLUSION: The induction of apoptosis in jurkat cells as well as the mean signal intensity, but not the mean number, of γ-H2AX foci per cell depends on the LET. |
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| 700 | 1 | _ | |a Giesen, Ulrich |0 P:(DE-HGF)0 |b 1 |
| 700 | 1 | _ | |a Kriehuber, Ralf |0 P:(DE-Juel1)133469 |b 2 |u fzj |
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