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@INPROCEEDINGS{Unverricht:841253,
      author       = {Unverricht, Marcus and Giesen, Ulrich and Pomplun, Ekkehard
                      and Kriehuber, Ralf},
      title        = {{D}oes gene expression analysis allow the discrimination of
                      radiation qualities?},
      reportid     = {FZJ-2017-08345},
      year         = {2015},
      abstract     = {Gene expression analysis was carried out in human
                      T-lymphoma Jurkat cells in order to identify robust
                      candidate genes showing significant gene expression
                      alterations after exposure to I-123-iododeoxyuridine,
                      γ-rays and α-particles. Equi-effect radiation doses, i.e.
                      radiation doses and exposure conditions causing the same
                      biological effect level, were determined with regard to
                      micronucleus formation, γ-H2AX foci signal intensity and
                      apoptosis induction after γ-irradiation (Cs-137, 0.7
                      Gy/min), α-irradiation (Am-241, 0.032 Gy/min) and exposure
                      to the Auger electron emitter I-123 as
                      I-123-iododeoxyuridine (I-123-UdR). I-123-UdR was
                      incorporated into the DNA for 20 h. Absorbed radiation dose
                      was assessed based on accumulated decays, point-kernel
                      calculations and the 3-D morphology of the cells. Gene
                      expression analysis was performed employing whole human
                      genome DNA-microarrays (Agilent) after exposure to
                      equi-effect radiation doses. RNA for gene expression
                      analysis was isolated 6 and 24 h post-exposure. Potential
                      candidate genes for the discrimination of radiation quality
                      have to show a significant expression change (>1.5 fold;
                      p<0.05) and display no altered gene regulation or even a
                      conversely regulation in response to exposure to the other
                      radiation qualities investigated. Gene expression of
                      selected candidate genes was validated via qRT-PCR.
                      Biological processes and pathways of significantly regulated
                      genes were subsequently analyzed. At equi-effect doses 359,
                      598 and 1339 genes were significantly regulated after
                      exposure to I-123-UdR, α-particles and γ-rays,
                      respectively. Applying the above described requirements for
                      candidate genes, we identified 4, 1 and 1 gene(s) allowing
                      the reliable and robust discrimination between γ- vs.
                      I-123-UdR-exposure, γ- vs. α-radiation and α- vs.
                      I-123-UdR-exposure, respectively.The results indicate that
                      the discrimination of different radiation qualities by means
                      of gene expression is possible.Funded by Bundesministerium
                      für Bildung und Forschung (BMBF), Grant 02NUK005A},
      month         = {May},
      date          = {2015-05-23},
      organization  = {15th International Congress of
                       Radiation Research, Kyoto (Japan), 23
                       May 2015 - 29 May 2015},
      subtyp        = {After Call},
      cin          = {S-US},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)6},
      url          = {https://juser.fz-juelich.de/record/841253},
}