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| 001 | 841253 | ||
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| 100 | 1 | _ | |a Unverricht, Marcus |0 P:(DE-Juel1)133466 |b 0 |e Corresponding author |u fzj |
| 111 | 2 | _ | |a 15th International Congress of Radiation Research |g ICRR |c Kyoto |d 2015-05-23 - 2015-05-29 |w Japan |
| 245 | _ | _ | |a Does gene expression analysis allow the discrimination of radiation qualities? |
| 260 | _ | _ | |c 2015 |
| 336 | 7 | _ | |a Conference Paper |0 33 |2 EndNote |
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| 520 | _ | _ | |a Gene expression analysis was carried out in human T-lymphoma Jurkat cells in order to identify robust candidate genes showing significant gene expression alterations after exposure to I-123-iododeoxyuridine, γ-rays and α-particles. Equi-effect radiation doses, i.e. radiation doses and exposure conditions causing the same biological effect level, were determined with regard to micronucleus formation, γ-H2AX foci signal intensity and apoptosis induction after γ-irradiation (Cs-137, 0.7 Gy/min), α-irradiation (Am-241, 0.032 Gy/min) and exposure to the Auger electron emitter I-123 as I-123-iododeoxyuridine (I-123-UdR). I-123-UdR was incorporated into the DNA for 20 h. Absorbed radiation dose was assessed based on accumulated decays, point-kernel calculations and the 3-D morphology of the cells. Gene expression analysis was performed employing whole human genome DNA-microarrays (Agilent) after exposure to equi-effect radiation doses. RNA for gene expression analysis was isolated 6 and 24 h post-exposure. Potential candidate genes for the discrimination of radiation quality have to show a significant expression change (>1.5 fold; p<0.05) and display no altered gene regulation or even a conversely regulation in response to exposure to the other radiation qualities investigated. Gene expression of selected candidate genes was validated via qRT-PCR. Biological processes and pathways of significantly regulated genes were subsequently analyzed. At equi-effect doses 359, 598 and 1339 genes were significantly regulated after exposure to I-123-UdR, α-particles and γ-rays, respectively. Applying the above described requirements for candidate genes, we identified 4, 1 and 1 gene(s) allowing the reliable and robust discrimination between γ- vs. I-123-UdR-exposure, γ- vs. α-radiation and α- vs. I-123-UdR-exposure, respectively.The results indicate that the discrimination of different radiation qualities by means of gene expression is possible.Funded by Bundesministerium für Bildung und Forschung (BMBF), Grant 02NUK005A |
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| 700 | 1 | _ | |a Kriehuber, Ralf |0 P:(DE-Juel1)133469 |b 3 |u fzj |
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