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@INPROCEEDINGS{Unverricht:841256,
      author       = {Unverricht, Marcus and Giesen, Ulrich and Pomplun, Ekkehard
                      and Kriehuber, Ralf},
      title        = {{G}enotoxicity of the {A}uger electron emitter
                      {I}-123-iododeoxyuridine in comparison to high- and
                      low-{LET} radiation},
      reportid     = {FZJ-2017-08348},
      year         = {2015},
      abstract     = {The biological effectiveness of Auger electron emitters
                      (AEE) is attributed to the numerous short-range electrons
                      released during the decay of these radionuclides. Damage on
                      cellular level depends largely on their intracellular
                      distribution. AEE located exclusively in the cytoplasm
                      produce low-LET type cell survival curves, whereas
                      DNA-associated AEE cause high-LET type survival curves. To
                      determine whether AEE induce high-LET type genotoxic
                      effects, micronucleus induction and γ-H2AX formation were
                      analyzed after exposure to I-123-iododeoxyuridine
                      (I-123-UdR) in comparison to high- and low-LET
                      radiation.Human T-lymphoma Jurkat cells were either exposed
                      to I-123-UdR for 20 h or irradiated with different doses of
                      low-LET γ-rays (Cs-137, 0.7 Gy/min) or high-LET
                      α-particles (Am-241, 0.032 Gy/min). Cells were assayed for
                      micronucleus formation (Cytochalasin B assay) employing
                      automated image analysis (MetaSystems, Germany). The γ-H2AX
                      foci were quantified by measuring the mean signal intensity
                      of γ-H2AX foci per cell using flow cytometry and by
                      counting the number of γ-H2AX foci with a fluorescence
                      microscope.In contrast to γ- and α-irradiation the numbers
                      of γ-H2AX foci per cell showed a much more pronounced
                      increase after exposure to I-123-UdR. However, the mean
                      intensity of γ H2AX signals per cell, as measured by flow
                      cytometry, was very similar for exposure to I-123-UdR and
                      α-particles. Single γ H2AX foci induced by I-123-UdR
                      appear to be smaller and/or less intense stained than those
                      after α-irradiation and resemble γ H2AX foci induced by
                      γ-rays. Micronucleus induction was almost identical for all
                      three investigated radiation qualities.Due to the fact that
                      most of the ionizing events of I-123-UdR occurred within the
                      DNA, γ H2AX foci are very efficiently induced by I-123-UdR
                      when compared to γ- and α-radiation. Taken into account
                      the very low dose rate of I-123-UdR exposure, the effect is
                      even more pronounced. The presumed complexity of the
                      DNA-lesions caused by DNA-associated AEE is not reflected in
                      the size and the intensity of γ-H2AX foci. Funded by
                      Bundesministerium für Bildung und Forschung (BMBF), Grant
                      No.: 02NUK005A},
      month         = {May},
      date          = {2015-05-23},
      organization  = {15th International Congress of
                       Radiation Research, Kyoto (Japan), 23
                       May 2015 - 29 May 2015},
      subtyp        = {After Call},
      cin          = {S-US},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)24},
      url          = {https://juser.fz-juelich.de/record/841256},
}