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@INPROCEEDINGS{Unverricht:841261,
      author       = {Unverricht, Marcus and Giesen, Ulrich and Pomplun, Ekkehard
                      and Kriehuber, Ralf},
      title        = {{C}omparative analysis of gene expression data after
                      exposure to {I}odine-123 labeled 5-{I}odo-2´-deoxyuridine,
                      γ-rays and α-particles},
      reportid     = {FZJ-2017-08353},
      year         = {2015},
      abstract     = {To investigate whether exposure to different radiation
                      qualities is reflected in a significant differentially gene
                      expression respective analysis were carried out in human
                      T-lymphoma Jurkat cells after exposure to Iodine-123 labeled
                      5-Iodo-2´-deoxyuridine (I-123-UdR), γ-rays and
                      α-particles. Potential gene markers were
                      identified.Equi-effect radiation doses, i.e. radiation doses
                      and exposure conditions causing the same biological effect
                      level, were determined in human T-lymphoma Jurkat cells with
                      regard to micronucleus formation, γ-H2AX foci signal
                      intensity and apoptosis induction after γ-irradiation
                      (Cs-137, 0.7 Gy/min), α-irradiation (Am-241, 0.032 Gy/min)
                      and exposure to the Auger electron emitter I-123 which was
                      incorporated as I-123-UdR into the DNA for 20 h. Radiation
                      dose for I-123 exposure was assessed by point-kernel
                      calculations and 3-D morphology of the cells. Whole human
                      genome DNA-microarrays (Agilent) were employed to measure
                      gene expression after exposure to equi-effect doses. RNA was
                      isolated 6 and 24 h post-exposure. The criteria for
                      candidate genes were a significant expression change (>1.5
                      fold; p<0.05) and no altered or even a conversely regulation
                      in response to the other radiation qualities. Expression of
                      selected candidate genes was validated via qRT-PCR.
                      Biological processes and pathways of significantly regulated
                      genes were subsequently analyzed. At equi-effect doses 1055,
                      318 and 165 genes were exclusively regulated after exposure
                      to γ-rays, α-particles and I-123-UdR, respectively. The
                      biological processes Apoptosis and Nucleosome Organization
                      were activated. According to the strict requirements for
                      potential gene markers, we identified 4, 1 and 1 gene(s)
                      allowing a robust discrimination between γ- vs.
                      I-123-UdR-exposure, γ- vs. α-radiation and α- vs.
                      I-123-UdR-exposure, respectively. The presented results
                      indicate that gene expression analysis might be an effective
                      tool for the discrimination between high- and low-LET
                      radiation. In addition, it seems to be possible to
                      distinguish between different types of high-LET
                      radiation.Funded by Bundesministerium für Bildung und
                      Forschung (BMBF), Grant 02NUK005A},
      month         = {May},
      date          = {2015-05-20},
      organization  = {8th International Symposium On
                       Physical, Molecular, Cellular, And
                       Medical Aspects Of Auger Processes,
                       Nara (Japan), 20 May 2015 - 22 May
                       2015},
      subtyp        = {After Call},
      cin          = {S-US},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)6},
      url          = {https://juser.fz-juelich.de/record/841261},
}