Gast ::
| Hauptseite > Publikationsdatenbank > Comparative analysis of gene expression data after exposure to Iodine-123 labeled 5-Iodo-2´-deoxyuridine, γ-rays and α-particles > print |
| Hauptseite > Publikationsdatenbank > Comparative analysis of gene expression data after exposure to Iodine-123 labeled 5-Iodo-2´-deoxyuridine, γ-rays and α-particles > print |
| 001 | 841261 | ||
| 005 | 20210129232005.0 | ||
| 024 | 7 | _ | |a 2128/16220 |2 Handle |
| 037 | _ | _ | |a FZJ-2017-08353 |
| 041 | _ | _ | |a English |
| 100 | 1 | _ | |a Unverricht, Marcus |0 P:(DE-Juel1)133466 |b 0 |e Corresponding author |u fzj |
| 111 | 2 | _ | |a 8th International Symposium On Physical, Molecular, Cellular, And Medical Aspects Of Auger Processes |g ISPMC |c Nara |d 2015-05-20 - 2015-05-22 |w Japan |
| 245 | _ | _ | |a Comparative analysis of gene expression data after exposure to Iodine-123 labeled 5-Iodo-2´-deoxyuridine, γ-rays and α-particles |
| 260 | _ | _ | |c 2015 |
| 336 | 7 | _ | |a Conference Paper |0 33 |2 EndNote |
| 336 | 7 | _ | |a Other |2 DataCite |
| 336 | 7 | _ | |a INPROCEEDINGS |2 BibTeX |
| 336 | 7 | _ | |a conferenceObject |2 DRIVER |
| 336 | 7 | _ | |a LECTURE_SPEECH |2 ORCID |
| 336 | 7 | _ | |a Conference Presentation |b conf |m conf |0 PUB:(DE-HGF)6 |s 1513178281_1261 |2 PUB:(DE-HGF) |x After Call |
| 520 | _ | _ | |a To investigate whether exposure to different radiation qualities is reflected in a significant differentially gene expression respective analysis were carried out in human T-lymphoma Jurkat cells after exposure to Iodine-123 labeled 5-Iodo-2´-deoxyuridine (I-123-UdR), γ-rays and α-particles. Potential gene markers were identified.Equi-effect radiation doses, i.e. radiation doses and exposure conditions causing the same biological effect level, were determined in human T-lymphoma Jurkat cells with regard to micronucleus formation, γ-H2AX foci signal intensity and apoptosis induction after γ-irradiation (Cs-137, 0.7 Gy/min), α-irradiation (Am-241, 0.032 Gy/min) and exposure to the Auger electron emitter I-123 which was incorporated as I-123-UdR into the DNA for 20 h. Radiation dose for I-123 exposure was assessed by point-kernel calculations and 3-D morphology of the cells. Whole human genome DNA-microarrays (Agilent) were employed to measure gene expression after exposure to equi-effect doses. RNA was isolated 6 and 24 h post-exposure. The criteria for candidate genes were a significant expression change (>1.5 fold; p<0.05) and no altered or even a conversely regulation in response to the other radiation qualities. Expression of selected candidate genes was validated via qRT-PCR. Biological processes and pathways of significantly regulated genes were subsequently analyzed. At equi-effect doses 1055, 318 and 165 genes were exclusively regulated after exposure to γ-rays, α-particles and I-123-UdR, respectively. The biological processes Apoptosis and Nucleosome Organization were activated. According to the strict requirements for potential gene markers, we identified 4, 1 and 1 gene(s) allowing a robust discrimination between γ- vs. I-123-UdR-exposure, γ- vs. α-radiation and α- vs. I-123-UdR-exposure, respectively. The presented results indicate that gene expression analysis might be an effective tool for the discrimination between high- and low-LET radiation. In addition, it seems to be possible to distinguish between different types of high-LET radiation.Funded by Bundesministerium für Bildung und Forschung (BMBF), Grant 02NUK005A |
| 536 | _ | _ | |a 899 - ohne Topic (POF3-899) |0 G:(DE-HGF)POF3-899 |c POF3-899 |f POF III |x 0 |
| 650 | 2 | 7 | |a Biology |0 V:(DE-MLZ)SciArea-160 |2 V:(DE-HGF) |x 0 |
| 700 | 1 | _ | |a Giesen, Ulrich |0 P:(DE-HGF)0 |b 1 |
| 700 | 1 | _ | |a Pomplun, Ekkehard |0 P:(DE-Juel1)133341 |b 2 |
| 700 | 1 | _ | |a Kriehuber, Ralf |0 P:(DE-Juel1)133469 |b 3 |u fzj |
| 856 | 4 | _ | |y OpenAccess |u https://juser.fz-juelich.de/record/841261/files/Nara%2C%20Japan%202015%20oral%20presentation.pdf |
| 856 | 4 | _ | |y OpenAccess |x icon |u https://juser.fz-juelich.de/record/841261/files/Nara%2C%20Japan%202015%20oral%20presentation.gif?subformat=icon |
| 856 | 4 | _ | |y OpenAccess |x icon-1440 |u https://juser.fz-juelich.de/record/841261/files/Nara%2C%20Japan%202015%20oral%20presentation.jpg?subformat=icon-1440 |
| 856 | 4 | _ | |y OpenAccess |x icon-180 |u https://juser.fz-juelich.de/record/841261/files/Nara%2C%20Japan%202015%20oral%20presentation.jpg?subformat=icon-180 |
| 856 | 4 | _ | |y OpenAccess |x icon-640 |u https://juser.fz-juelich.de/record/841261/files/Nara%2C%20Japan%202015%20oral%20presentation.jpg?subformat=icon-640 |
| 856 | 4 | _ | |y OpenAccess |x pdfa |u https://juser.fz-juelich.de/record/841261/files/Nara%2C%20Japan%202015%20oral%20presentation.pdf?subformat=pdfa |
| 909 | C | O | |o oai:juser.fz-juelich.de:841261 |p openaire |p open_access |p VDB |p driver |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 0 |6 P:(DE-Juel1)133466 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 3 |6 P:(DE-Juel1)133469 |
| 913 | 1 | _ | |a DE-HGF |b Programmungebundene Forschung |l ohne Programm |1 G:(DE-HGF)POF3-890 |0 G:(DE-HGF)POF3-899 |2 G:(DE-HGF)POF3-800 |v ohne Topic |x 0 |4 G:(DE-HGF)POF |3 G:(DE-HGF)POF3 |
| 915 | _ | _ | |a OpenAccess |0 StatID:(DE-HGF)0510 |2 StatID |
| 920 | _ | _ | |l yes |
| 920 | 1 | _ | |0 I:(DE-Juel1)S-US-20090406 |k S-US |l Sicherheit und Strahlenschutz, Umgebungsüberwachung,Strahlenbiologie |x 0 |
| 980 | _ | _ | |a conf |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a UNRESTRICTED |
| 980 | _ | _ | |a I:(DE-Juel1)S-US-20090406 |
| 980 | 1 | _ | |a FullTexts |
| Library | Collection | CLSMajor | CLSMinor | Language | Author |
|---|